Fitness for 5 genes in Methanococcus maripaludis JJ

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500 ntMMJJ_RS01740 and MMJJ_RS01745 are separated by 181 nucleotidesMMJJ_RS01745 and MMJJ_RS01750 are separated by 111 nucleotidesMMJJ_RS01750 and MMJJ_RS09260 are separated by 114 nucleotidesMMJJ_RS09260 and MMJJ_RS01755 are separated by 15 nucleotides MMJJ_RS01740: MMJJ_RS01740 - ATP-binding protein, at 336,892 to 338,058 _RS01740 MMJJ_RS01745: MMJJ_RS01745 - metal-dependent transcriptional regulator, at 338,240 to 338,645 _RS01745 MMJJ_RS01750: MMJJ_RS01750 - ferrous iron transport protein A, at 338,757 to 338,963 _RS01750 MMJJ_RS09260: MMJJ_RS09260 - hypothetical protein, at 339,078 to 339,215 _RS09260 MMJJ_RS01755: MMJJ_RS01755 - ferrous iron transport protein B, at 339,231 to 341,174 _RS01755
Group Condition MMJJ_RS01740 MMJJ_RS01745 MMJJ_RS01750 MMJJ_RS09260 MMJJ_RS01755
nitrogen source H2 N-free with 2g/L casaminos 10mM Ammonium chloride +0.1 -0.5 -4.8 N.D. -3.7
nitrogen source H2 N-free with 2g/L casaminos -0.1 -0.2 -4.9 N.D. -3.7
attachment assay; attached sample McCas-formate glass attached -0.0 -0.0 -4.6 N.D. -4.1
nitrogen source H2 N-free with 10mM Ammonium chloride -0.1 -0.1 -4.7 N.D. -3.6
iron Iron excess (2.5x) , H2 media -0.1 -0.2 -4.8 N.D. -3.2
hydrogen no stress control H2 no stress control +0.1 -0.1 -4.3 N.D. -4.0
iron Iron limitation (0.2x), H2 media -0.0 -0.1 -4.3 N.D. -3.8
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE +0.1 +0.0 -4.4 N.D. -3.8
formate no stress control Formate no stress control -0.0 +0.2 -4.6 N.D. -3.7
iron control, 1x iron with casaminos and vitamins, H2 media +0.1 -0.2 -4.8 N.D. -3.2
nutrient t1 formate McAA biotin dropout -0.1 +0.1 -4.3 N.D. -3.8
nutrient t1 formate McAA pyroxidine HCl dropout -0.2 -0.4 -4.7 N.D. -2.7
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE +0.1 -0.1 -4.3 N.D. -3.7
iron Iron excess (2.5x) , H2 media -0.1 -0.3 -4.2 N.D. -3.3
nutrient t1 formate McAA pyroxidine HCl dropout -0.1 +0.1 -4.2 N.D. -3.8
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE +0.1 -0.0 -4.3 N.D. -3.7
iron control, 1x iron with casaminos and vitamins, H2 media -0.0 -0.3 -4.8 N.D. -2.8
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE +0.2 -0.1 -4.3 N.D. -3.7
nitrogen source H2 N-free with 2g/L casaminos -0.1 -0.3 -3.8 N.D. -3.7
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Casamino Acid FREE -0.0 -0.0 -4.2 N.D. -3.6
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 +0.1 -0.1 -4.3 N.D. -3.7
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 +0.0 -0.1 -3.9 N.D. -3.8
nutrient t1 formate McAA pantothenate dropout -0.1 +0.2 -4.3 N.D. -3.5
iron Iron limitation (0.2x), formate media -0.0 +0.2 -4.3 N.D. -3.5
nutrient t1 formate McAA B12 dropout -0.2 +0.2 -4.2 N.D. -3.4
nutrient t1 formate McAA p-aminobenzoic dropout -0.0 +0.2 -3.9 N.D. -3.9
nutrient t1 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 Vitamin & Casamino Acid FREE -0.1 +0.2 -3.9 N.D. -3.8
nutrient t1 formate McAA thiamine dropout -0.3 +0.4 -3.9 N.D. -3.8
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE +0.2 +0.1 -4.1 N.D. -3.5
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE +0.2 +0.3 -4.2 N.D. -3.6
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