Fitness for 5 genes in Methanococcus maripaludis JJ

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500 ntMMJJ_RS01680 and MMJJ_RS01685 are separated by 4 nucleotidesMMJJ_RS01685 and MMJJ_RS01690 are separated by 142 nucleotidesMMJJ_RS01690 and MMJJ_RS01695 are separated by 147 nucleotidesMMJJ_RS01695 and MMJJ_RS01700 are separated by 39 nucleotides MMJJ_RS01680: MMJJ_RS01680 - cation diffusion facilitator family transporter, at 325,625 to 326,500 _RS01680 MMJJ_RS01685: MMJJ_RS01685 - DUF354 domain-containing protein, at 326,505 to 327,518 _RS01685 MMJJ_RS01690: MMJJ_RS01690 - methanogenesis marker 12 protein, at 327,661 to 328,638 _RS01690 MMJJ_RS01695: MMJJ_RS01695 - 50S ribosomal protein L7Ae, at 328,786 to 329,139 _RS01695 MMJJ_RS01700: MMJJ_RS01700 - 30S ribosomal protein S28e, at 329,179 to 329,409 _RS01700
Group Condition MMJJ_RS01680 MMJJ_RS01685 MMJJ_RS01690 MMJJ_RS01695 MMJJ_RS01700
rich t2 H2 McCas, 5g/L bicarb, 40 psi H2CO2 -2.4 N.D. -3.5 N.D. N.D.
iron Iron excess (2.5x) , H2 media +0.0 N.D. -4.4 N.D. N.D.
biofilm H2 pooled triplicate biofilm cells -0.5 N.D. -3.7 N.D. N.D.
iron control, 1x iron with casaminos and vitamins, formate media -0.3 N.D. -3.6 N.D. N.D.
nutrient t1 formate McAA pyroxidine HCl dropout -0.4 N.D. -3.4 N.D. N.D.
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE -0.3 N.D. -3.5 N.D. N.D.
nitrogen source H2 N-free with 2g/L casaminos 10mM Ammonium chloride -0.2 N.D. -3.4 N.D. N.D.
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE -1.1 N.D. -2.5 N.D. N.D.
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 -0.3 N.D. -3.2 N.D. N.D.
iron control, 1x iron with casaminos and vitamins, H2 media -0.0 N.D. -3.4 N.D. N.D.
stress McCas_formate_complete Chlorite 0.3125 mM -0.4 N.D. -3.1 N.D. N.D.
nutrient t1 formate McAA biotin dropout -0.2 N.D. -3.2 N.D. N.D.
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE -0.1 N.D. -3.4 N.D. N.D.
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE -1.1 N.D. -2.3 N.D. N.D.
nitrogen source H2 N-free with 10mM Ammonium chloride -0.2 N.D. -3.2 N.D. N.D.
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE -1.2 N.D. -2.2 N.D. N.D.
iron Iron excess (2.5x) , H2 media -0.1 N.D. -3.3 N.D. N.D.
nitrogen source H2 N-free with 2g/L casaminos 10mM Ammonium chloride -0.0 N.D. -3.3 N.D. N.D.
stress McCas_formate_complete Polymyxin B sulfate 0.05 mM -0.6 N.D. -2.7 N.D. N.D.
nutrient t1 formate McAA lipolic dropout -0.2 N.D. -3.0 N.D. N.D.
nutrient t1 formate McAA B12 dropout -0.4 N.D. -2.8 N.D. N.D.
nutrient t1 formate McAA thiamine dropout -0.4 N.D. -2.8 N.D. N.D.
no stress control McCas_formate_complete no stress control -0.2 N.D. -3.0 N.D. N.D.
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE -0.1 N.D. -3.1 N.D. N.D.
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE -0.6 N.D. -2.6 N.D. N.D.
no stress control McCas_formate_complete no stress control -0.2 N.D. -2.9 N.D. N.D.
stress McCas_formate_complete sodium bromide 125 mM -0.9 N.D. -2.2 N.D. N.D.
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE -0.8 N.D. -2.4 N.D. N.D.
stress McCas_formate_complete silver chloride 0.125 mM -0.2 N.D. -2.9 N.D. N.D.
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Casamino Acid FREE +0.3 N.D. -3.0 N.D. N.D.
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