Fitness for 5 genes in Methanococcus maripaludis JJ

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500 ntMMJJ_RS01590 and MMJJ_RS01595 are separated by 42 nucleotidesMMJJ_RS01595 and MMJJ_RS01600 are separated by 91 nucleotidesMMJJ_RS01600 and MMJJ_RS01605 are separated by 101 nucleotidesMMJJ_RS01605 and MMJJ_RS01610 are separated by 75 nucleotides MMJJ_RS01590: MMJJ_RS01590 - adenine phosphoribosyltransferase, at 309,534 to 310,052 _RS01590 MMJJ_RS01595: MMJJ_RS01595 - U32 family peptidase, at 310,095 to 311,306 _RS01595 MMJJ_RS01600: MMJJ_RS01600 - radical SAM protein, at 311,398 to 312,291 _RS01600 MMJJ_RS01605: MMJJ_RS01605 - ACT domain-containing protein, at 312,393 to 312,665 _RS01605 MMJJ_RS01610: MMJJ_RS01610 - methanogenesis marker 17 protein, at 312,741 to 313,292 _RS01610
Group Condition MMJJ_RS01590 MMJJ_RS01595 MMJJ_RS01600 MMJJ_RS01605 MMJJ_RS01610
nitrogen source H2 N-free with 10mM Ammonium chloride -0.6 -0.3 -4.7 N.D. N.D.
nutrient t1 formate vitamin complete -0.4 -0.1 -4.9 N.D. N.D.
iron control, 1x iron with casaminos and vitamins, H2 media -0.6 -0.1 -4.6 N.D. N.D.
iron Iron excess (2.5x) , H2 media -0.5 -0.0 -4.6 N.D. N.D.
iron Iron excess (2.5x) , H2 media -0.2 -0.2 -4.6 N.D. N.D.
iron Iron excess (2.5x) , H2 media +0.0 -0.4 -4.6 N.D. N.D.
nitrogen source H2 N-free with 2g/L casaminos 10mM Ammonium chloride +0.1 -0.2 -4.7 N.D. N.D.
iron control, 1x iron with casaminos and vitamins, H2 media -0.0 -0.3 -4.6 N.D. N.D.
nitrogen source H2 N-free with 2g/L casaminos +0.0 -0.1 -4.7 N.D. N.D.
iron Iron limitation (0.2x), H2 media -0.9 -0.2 -3.6 N.D. N.D.
iron Iron limitation (0.2x), formate media +0.0 +0.1 -4.9 N.D. N.D.
nutrient t1 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 Vitamin & Casamino Acid FREE -0.3 -0.2 -4.2 N.D. N.D.
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE -0.1 -0.5 -4.0 N.D. N.D.
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE -0.2 -0.5 -3.9 N.D. N.D.
nutrient t1 formate McAA pyroxidine HCl dropout -0.3 +0.1 -4.4 N.D. N.D.
nitrogen source H2 N-free with 2g/L casaminos 10mM Ammonium chloride +0.3 -0.1 -4.6 N.D. N.D.
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 -0.0 -0.4 -4.1 N.D. N.D.
nutrient t1 formate McAA p-aminobenzoic dropout -0.2 +0.1 -4.2 N.D. N.D.
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 +0.3 -0.9 -3.7 N.D. N.D.
nutrient t1 formate McAA pantothenate dropout +0.6 +0.0 -4.9 N.D. N.D.
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE +0.3 -0.6 -3.9 N.D. N.D.
nutrient t1 formate McAA lipolic dropout +0.7 +0.0 -4.9 N.D. N.D.
rich t1 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 FREE +0.6 -0.3 -4.2 N.D. N.D.
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE +0.5 -0.4 -3.8 N.D. N.D.
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE +0.5 -0.3 -3.8 N.D. N.D.
nutrient t1 formate McAA thiamine dropout +0.3 +0.3 -3.9 N.D. N.D.
nitrogen source Formate N-free with 2mM Adenosine +1.9 +0.1 -3.5 N.D. N.D.
nitrogen source Formate N-free with 2mM Adenosine +3.0 -0.1 -3.3 N.D. N.D.
nitrogen source H2 N-free with 2mM Adenosine +3.2 -0.3 -2.1 N.D. N.D.
nitrogen source H2 N-free with 2mM Adenosine +3.1 -0.6 -1.5 N.D. N.D.
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