Fitness for 5 genes in Methanococcus maripaludis JJ

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500 ntMMJJ_RS01190 and MMJJ_RS01195 are separated by 76 nucleotidesMMJJ_RS01195 and MMJJ_RS01200 are separated by 24 nucleotidesMMJJ_RS01200 and MMJJ_RS09335 are separated by 125 nucleotidesMMJJ_RS09335 and MMJJ_RS01205 are separated by 28 nucleotides MMJJ_RS01190: MMJJ_RS01190 - hypothetical protein, at 228,923 to 229,852 _RS01190 MMJJ_RS01195: MMJJ_RS01195 - aspartate dehydrogenase, at 229,929 to 230,732 _RS01195 MMJJ_RS01200: MMJJ_RS01200 - PRC-barrel domain-containing protein, at 230,757 to 231,011 _RS01200 MMJJ_RS09335: MMJJ_RS09335 - hypothetical protein, at 231,137 to 231,307 _RS09335 MMJJ_RS01205: MMJJ_RS01205 - DNA adenine methylase, at 231,336 to 232,256 _RS01205
Group Condition MMJJ_RS01190 MMJJ_RS01195 MMJJ_RS01200 MMJJ_RS09335 MMJJ_RS01205
nutrient t1 formate McAA lipolic dropout -0.1 N.D. -3.5 -0.6 -0.2
nutrient t1 formate vitamin complete -0.2 N.D. -3.4 -0.5 -0.2
biofilm Formate pooled triplicate biofilm cells -0.1 N.D. -3.6 -0.2 -0.1
biofilm H2 pooled triplicate biofilm cells -0.2 N.D. -2.9 -0.6 -0.4
nitrogen source H2 N-free with 2g/L casaminos 10mM Ammonium chloride -0.2 N.D. -3.4 -0.2 -0.1
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE -0.1 N.D. -3.4 -0.3 +0.0
nutrient t1 formate McAA thiamine dropout -0.3 N.D. -3.1 -0.1 -0.2
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE -0.3 N.D. -2.4 -0.5 -0.5
nitrogen source H2 N-free with 2g/L casaminos -0.1 N.D. -3.5 -0.0 -0.0
stress Formate 100 mM hypophosphite -0.0 N.D. -3.6 +0.1 -0.1
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE -0.3 N.D. -2.4 -0.5 -0.4
nutrient t1 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 Vitamin & Casamino Acid FREE -0.0 N.D. -3.3 -0.3 +0.1
nitrogen source H2 N-free with 2g/L casaminos 10mM Ammonium chloride -0.1 N.D. -3.3 -0.1 -0.1
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE -0.4 N.D. -2.2 -0.5 -0.4
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 +0.0 N.D. -3.3 -0.4 +0.2
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Casamino Acid FREE +0.1 N.D. -3.2 -0.5 +0.2
stress McCas_formate_complete Sulfaquinoxaline 0.1 mM -0.2 N.D. -2.6 -0.3 -0.3
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE -0.2 N.D. -3.4 +0.0 +0.1
planktonic H2 pooled triplicate planktonic cells -0.2 N.D. -3.1 +0.2 -0.3
iron Iron excess (2.5x) , H2 media +0.1 N.D. -3.5 +0.2 -0.2
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE -0.1 N.D. -2.9 -0.7 +0.4
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE -0.1 N.D. -3.0 -0.4 +0.2
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE -0.1 N.D. -2.8 -0.5 +0.2
stress McCas_formate_complete Polymyxin B sulfate 0.05 mM -0.4 N.D. -2.8 +0.1 -0.1
iron Iron limitation (0.2x), H2 media -0.2 N.D. -3.1 +0.2 +0.0
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 +0.0 N.D. -3.1 -0.2 +0.2
stress McCas_formate_complete selenate 31.25 mM -0.3 N.D. -2.3 -0.7 +0.4
iron Iron excess (2.5x) , H2 media -0.2 N.D. -2.6 -0.5 +0.4
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 -0.1 N.D. -2.7 -0.2 +0.4
iron Iron excess (2.5x) , H2 media -0.4 N.D. -2.8 +0.2 +0.3
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MMJJ_RS01190
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MMJJ_RS01195
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MMJJ_RS09335
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MMJJ_RS01205
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