Fitness for 5 genes in Methanococcus maripaludis JJ

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500 ntMMJJ_RS00590 and MMJJ_RS00595 are separated by 13 nucleotidesMMJJ_RS00595 and MMJJ_RS00600 are separated by 89 nucleotidesMMJJ_RS00600 and MMJJ_RS00605 are separated by 122 nucleotidesMMJJ_RS00605 and MMJJ_RS00610 are separated by 40 nucleotides MMJJ_RS00590: MMJJ_RS00590 - class I SAM-dependent methyltransferase, at 111,367 to 112,227 _RS00590 MMJJ_RS00595: MMJJ_RS00595 - tetratricopeptide repeat protein, at 112,241 to 113,956 _RS00595 MMJJ_RS00600: MMJJ_RS00600 - hydroxymethylbilane synthase, at 114,046 to 114,960 _RS00600 MMJJ_RS00605: MMJJ_RS00605 - ABC transporter permease, at 115,083 to 116,276 _RS00605 MMJJ_RS00610: MMJJ_RS00610 - D-2-hydroxyacid dehydrogenase, at 116,317 to 117,270 _RS00610
Group Condition MMJJ_RS00590 MMJJ_RS00595 MMJJ_RS00600 MMJJ_RS00605 MMJJ_RS00610
iron Iron limitation (0.2x), H2 media -0.0 -0.2 -5.6 -0.0 -0.1
iron Iron excess (2.5x) , H2 media +0.0 -0.3 -5.4 -0.1 +0.1
hydrogen no stress control H2 no stress control +0.0 +0.0 -5.4 -0.0 -0.1
rich t2 H2 McCas, 5g/L bicarb, 40 psi H2CO2 -0.2 -0.5 -4.1 -0.5 -0.2
nitrogen source H2 N-free with 10mM Ammonium chloride -0.2 -0.1 -5.3 +0.3 +0.0
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE -0.1 -0.3 -4.6 -0.1 -0.2
iron Iron limitation (0.2x), H2 media -0.1 -0.1 -4.9 -0.1 -0.0
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE -0.1 -0.3 -4.3 -0.2 -0.2
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE -0.0 -0.3 -4.5 -0.2 -0.1
hydrogen no stress control H2 no stress control -0.0 +0.1 -5.0 -0.1 -0.1
nutrient t1 formate McAA lipolic dropout -0.1 -0.2 -4.5 -0.2 -0.0
nitrogen source H2 N-free with 2g/L casaminos +0.0 -0.3 -4.9 +0.2 +0.0
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 -0.1 -0.3 -4.4 -0.1 -0.1
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 -0.1 -0.2 -4.2 -0.3 -0.2
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 -0.0 -0.3 -4.6 +0.1 -0.1
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE +0.0 -0.5 -4.4 +0.0 -0.0
iron control, 1x iron with casaminos and vitamins, H2 media -0.1 -0.2 -4.7 +0.1 +0.1
nitrogen source H2 N-free with 2g/L casaminos -0.0 -0.2 -4.8 +0.2 +0.1
iron Iron excess (2.5x) , H2 media +0.0 -0.3 -4.7 +0.0 +0.1
nitrogen source H2 N-free with 10mM D-glucosamine -0.0 -0.1 -4.7 +0.1 +0.0
nutrient t1 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE -0.1 -0.1 -4.4 +0.1 -0.1
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 Vitamin & Casamino Acid FREE -0.1 -0.4 -3.0 -0.7 -0.3
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE -0.0 -0.3 -4.0 +0.0 -0.2
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Casamino Acid FREE -0.1 -0.2 -4.2 +0.2 +0.0
nutrient t1 formate McAA biotin dropout -0.1 -0.2 -3.8 -0.3 +0.1
stress McCas_formate_complete selenate 31.25 mM +0.0 -0.5 -3.3 -0.3 -0.2
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 Vitamin & Casamino Acid FREE -0.1 -0.4 -3.0 -0.4 -0.2
iron Iron excess (2.5x) , H2 media +0.0 -0.2 -4.1 +0.1 +0.1
iron control, 1x iron with casaminos and vitamins, H2 media -0.0 -0.2 -4.1 +0.4 +0.0
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Casamino Acid FREE -0.2 -0.3 -3.3 +0.5 -0.1
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