Fitness for 5 genes in Methanococcus maripaludis JJ

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500 ntMMJJ_RS00495 and MMJJ_RS00500 are separated by 163 nucleotidesMMJJ_RS00500 and MMJJ_RS00505 are separated by 10 nucleotidesMMJJ_RS00505 and MMJJ_RS00510 are separated by 231 nucleotidesMMJJ_RS00510 and MMJJ_RS00515 are separated by 197 nucleotides MMJJ_RS00495: MMJJ_RS00495 - CTP synthase (glutamine hydrolyzing), at 91,678 to 93,279 _RS00495 MMJJ_RS00500: MMJJ_RS00500 - GNAT family N-acetyltransferase, at 93,443 to 93,991 _RS00500 MMJJ_RS00505: MMJJ_RS00505 - 3'-5' exonuclease, at 94,002 to 94,577 _RS00505 MMJJ_RS00510: MMJJ_RS00510 - DEAD/DEAH box helicase, at 94,809 to 97,049 _RS00510 MMJJ_RS00515: MMJJ_RS00515 - cobalt transporter CbiM, at 97,247 to 97,885 _RS00515
Group Condition MMJJ_RS00495 MMJJ_RS00500 MMJJ_RS00505 MMJJ_RS00510 MMJJ_RS00515
nitrogen source Formate N-free with 2mM Adenosine N.D. -0.7 -0.6 -0.4 -3.2
nitrogen source Formate N-free with 2mM Adenosine N.D. -0.1 -0.4 -0.7 -2.8
iron Iron excess (2.5x) , H2 media N.D. -0.6 -0.2 -0.6 -2.1
nitrogen source Formate N-free with 2mM Adenosine N.D. +0.1 +0.1 -0.2 -3.4
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 Vitamin & Casamino Acid FREE N.D. -0.1 -0.1 +0.1 -3.2
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 Vitamin & Casamino Acid FREE N.D. +0.0 -0.0 -0.1 -3.1
planktonic H2 pooled triplicate planktonic cells N.D. -0.1 +0.2 -0.9 -2.1
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 Vitamin & Casamino Acid FREE N.D. +0.0 -0.0 +0.1 -3.0
iron Iron excess (2.5x) , H2 media N.D. -0.5 +0.0 -0.2 -2.1
iron Iron limitation (0.2x), formate media N.D. +0.1 -0.4 +0.1 -2.5
nutrient t1 formate McAA pyroxidine HCl dropout N.D. -0.2 -0.2 -0.1 -2.2
nitrogen source H2 N-free with 2mM Adenosine N.D. -0.2 -0.3 -1.0 -1.3
nutrient t1 formate McAA riboflavin dropout N.D. -0.1 -0.2 -0.1 -2.1
iron Iron limitation (0.2x), formate media N.D. +0.2 -0.3 +0.1 -2.4
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 Vitamin & Casamino Acid FREE N.D. -0.4 +0.0 -0.5 -1.6
biofilm H2 pooled triplicate biofilm cells N.D. -0.2 +0.1 -0.6 -1.6
nitrogen source formate N-free with 10mM Ammonium chloride N.D. +0.1 -0.2 -0.1 -2.1
nutrient t1 formate McAA biotin dropout N.D. +0.3 -0.2 -0.1 -2.3
iron Iron limitation (0.2x), formate media N.D. +0.3 -0.3 +0.1 -2.3
nutrient t1 formate McAA riboflavin dropout N.D. +0.1 -0.2 -0.1 -2.1
nutrient t1 formate McAA p-aminobenzoic dropout N.D. +0.1 -0.1 -0.1 -2.2
nutrient t1 formate McAA thiamine dropout N.D. +0.2 -0.3 -0.1 -2.0
nutrient t1 formate vitamin complete N.D. +0.3 -0.3 -0.1 -2.0
iron control, 1x iron with casaminos and vitamins, formate media N.D. +0.2 -0.2 +0.0 -2.1
nitrogen source formate N-free with 10mM Ammonium chloride N.D. +0.2 -0.2 -0.1 -2.0
nutrient t1 formate McAA nicotinic dropout N.D. +0.3 -0.3 -0.2 -1.9
nutrient t1 formate McAA pyroxidine HCl dropout N.D. +0.4 -0.2 -0.0 -1.9
nutrient t1 formate McAA folic dropout N.D. +0.4 -0.2 +0.0 -1.9
iron Iron excess (2.5x) , formate media N.D. +0.5 -0.3 -0.0 -1.7
nitrogen source N2 fixation, H2 media N.D. +0.9 -0.1 -0.5 +1.2
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