Fitness for 5 genes in Methanococcus maripaludis JJ

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500 ntMMJJ_RS00270 and MMJJ_RS00275 are separated by 53 nucleotidesMMJJ_RS00275 and MMJJ_RS00280 are separated by 16 nucleotidesMMJJ_RS00280 and MMJJ_RS00285 are separated by 5 nucleotidesMMJJ_RS00285 and MMJJ_RS00290 are separated by 275 nucleotides MMJJ_RS00270: MMJJ_RS00270 - adenosylcobinamide-GDP ribazoletransferase, at 52,504 to 53,277 _RS00270 MMJJ_RS00275: MMJJ_RS00275 - coenzyme F420-0:L-glutamate ligase, at 53,331 to 54,077 _RS00275 MMJJ_RS00280: MMJJ_RS00280 - shikimate dehydrogenase, at 54,094 to 54,930 _RS00280 MMJJ_RS00285: MMJJ_RS00285 - UPF0254 family protein, at 54,936 to 55,433 _RS00285 MMJJ_RS00290: MMJJ_RS00290 - CheF family chemotaxis protein, at 55,709 to 56,755 _RS00290
Group Condition MMJJ_RS00270 MMJJ_RS00275 MMJJ_RS00280 MMJJ_RS00285 MMJJ_RS00290
iron Iron excess (2.5x) , H2 media -5.0 -5.1 N.D. -2.1 -0.6
iron Iron limitation (0.2x), H2 media -5.2 -5.3 N.D. -1.8 -0.3
nutrient t1 formate McAA folic dropout -4.6 -5.6 N.D. -0.7 -0.6
iron Iron excess (2.5x) , H2 media -4.5 -4.3 N.D. -2.1 -0.4
nitrogen source H2 N-free with 2g/L casaminos 10mM Ammonium chloride -4.4 -4.4 N.D. -2.1 -0.2
rich t2 H2 McCas, 5g/L bicarb, 40 psi H2CO2 -3.6 -3.5 N.D. -4.2 +0.4
iron Iron limitation (0.2x), H2 media -4.3 -4.5 N.D. -1.8 -0.2
nitrogen source H2 N-free with 10mM Ammonium chloride -4.3 -4.3 N.D. -2.0 -0.2
hydrogen no stress control H2 no stress control -3.5 -5.8 N.D. -1.5 +0.0
nutrient t1 formate McAA lipolic dropout -3.9 -5.4 N.D. -0.8 -0.4
nitrogen source H2 N-free with 2g/L casaminos 10mM Ammonium chloride -3.0 -5.3 N.D. -1.9 -0.3
iron control, 1x iron with casaminos and vitamins, H2 media -3.3 -5.1 N.D. -1.8 -0.2
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 -4.2 -4.0 N.D. -1.9 +0.0
nitrogen source H2 N-free with 2g/L casaminos 10mM Ammonium chloride -4.5 -3.4 N.D. -1.8 -0.3
iron Iron excess (2.5x) , formate media -3.4 -5.3 N.D. -0.8 -0.4
nutrient t1 formate McAA pyroxidine HCl dropout -4.9 -4.2 N.D. -0.5 -0.3
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 -3.9 -3.8 N.D. -2.2 +0.2
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE -4.0 -3.8 N.D. -1.9 +0.1
stress McCas_formate_complete Ticarcillin 12.5 mM -3.5 -2.5 N.D. -4.1 +0.4
nutrient t1 formate McAA pyroxidine HCl dropout -4.1 -4.7 N.D. -0.6 -0.2
nutrient t1 formate McAA biotin dropout -5.1 -3.4 N.D. -0.6 -0.4
nutrient t1 formate McAA riboflavin dropout -3.6 -5.4 N.D. -0.6 -0.0
iron Iron limitation (0.2x), formate media -4.0 -4.6 N.D. -0.5 -0.3
nutrient t1 formate McAA pantothenate dropout -4.2 -4.7 N.D. -0.4 -0.2
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 Vitamin & Casamino Acid FREE -2.6 -4.3 N.D. -2.8 +0.1
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE -3.8 -3.7 N.D. -2.0 +0.1
iron Iron excess (2.5x) , H2 media -3.4 -3.9 N.D. -1.9 -0.3
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE -4.0 -3.2 N.D. -2.3 +0.1
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 Vitamin & Casamino Acid FREE -2.5 -3.8 N.D. -3.1 +0.3
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE -3.4 -3.9 N.D. -1.9 +0.2
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