Fitness for 5 genes in Methanococcus maripaludis JJ

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500 ntMMJJ_RS00120 and MMJJ_RS00125 are separated by 17 nucleotidesMMJJ_RS00125 and MMJJ_RS00130 are separated by 256 nucleotidesMMJJ_RS00130 and MMJJ_RS00135 are separated by 86 nucleotidesMMJJ_RS00135 and MMJJ_RS00140 are separated by 48 nucleotides MMJJ_RS00120: MMJJ_RS00120 - histidinol dehydrogenase, at 22,922 to 24,196 _RS00120 MMJJ_RS00125: MMJJ_RS00125 - KEOPS complex subunit Cgi121, at 24,214 to 24,645 _RS00125 MMJJ_RS00130: MMJJ_RS00130 - uroporphyrinogen-III C-methyltransferase, at 24,902 to 25,621 _RS00130 MMJJ_RS00135: MMJJ_RS00135 - FmdE family protein, at 25,708 to 26,286 _RS00135 MMJJ_RS00140: MMJJ_RS00140 - hypothetical protein, at 26,335 to 26,592 _RS00140
Group Condition MMJJ_RS00120 MMJJ_RS00125 MMJJ_RS00130 MMJJ_RS00135 MMJJ_RS00140
hydrogen no stress control H2 no stress control N.D. -4.6 N.D. +0.1 -0.0
nutrient t1 formate vitamin complete N.D. -3.9 N.D. -0.2 -0.1
nutrient t1 formate vitamin complete N.D. -3.9 N.D. -0.2 -0.1
nitrogen source formate N-free with 10mM Ammonium chloride N.D. -4.0 N.D. -0.1 -0.1
formate no stress control Formate no stress control N.D. -4.2 N.D. -0.0 +0.1
rich t2 H2 McCas, 5g/L bicarb, 40 psi H2CO2 N.D. -3.2 N.D. -0.6 -0.2
attachment assay; attached sample McCas-formate glass attached N.D. -4.0 N.D. -0.1 +0.0
nutrient t1 formate McAA biotin dropout N.D. -3.8 N.D. -0.2 +0.1
nitrogen source H2 N-free with 2g/L casaminos 10mM Ammonium chloride N.D. -3.9 N.D. -0.1 +0.1
nutrient t1 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE N.D. -3.8 N.D. +0.1 -0.3
rich t1 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 N.D. -3.8 N.D. +0.1 -0.2
iron Iron limitation (0.2x), formate media N.D. -3.4 N.D. -0.3 -0.1
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE N.D. -3.5 N.D. -0.1 -0.2
hydrogen no stress control H2 no stress control N.D. -3.6 N.D. -0.0 -0.2
nitrogen source H2 N-free with 2g/L casaminos N.D. -3.9 N.D. -0.2 +0.3
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE N.D. -3.6 N.D. +0.1 -0.2
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE N.D. -3.7 N.D. +0.1 -0.2
rich t1 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 FREE N.D. -3.5 N.D. +0.1 -0.3
stress McCas_formate_complete Sulfaquinoxaline 0.1 mM N.D. -3.3 N.D. +0.3 -0.7
iron Iron excess (2.5x) , formate media N.D. -3.9 N.D. +0.0 +0.2
nitrogen source H2 N-free with 10mM Ammonium chloride N.D. -3.7 N.D. -0.1 +0.2
rich t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 FREE N.D. -3.7 N.D. +0.2 -0.1
stress McCas_formate_complete Spiramycin 0.1 mM N.D. -3.4 N.D. +0.2 -0.3
iron Iron limitation (0.2x), H2 media N.D. -3.9 N.D. +0.1 +0.3
iron Iron excess (2.5x) , H2 media N.D. -3.6 N.D. -0.3 +0.6
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE N.D. -3.6 N.D. +0.2 +0.2
planktonic H2 pooled triplicate planktonic cells N.D. -3.5 N.D. +0.0 +0.4
iron Iron excess (2.5x) , H2 media N.D. -3.2 N.D. -0.2 +0.5
no stress control McCas_formate_complete no stress control N.D. -3.4 N.D. +0.4 +0.1
stress McCas_formate_complete deta nonoate 0.025 mM N.D. -3.5 N.D. +0.5 +0.3
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