Fitness for 5 genes in Rhodanobacter denitrificans FW104-10B01

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500 ntLRK54_RS17320 and LRK54_RS17325 are separated by 77 nucleotidesLRK54_RS17325 and LRK54_RS17330 are separated by 140 nucleotidesLRK54_RS17330 and LRK54_RS17335 overlap by 4 nucleotidesLRK54_RS17335 and LRK54_RS17340 are separated by 169 nucleotides LRK54_RS17320: LRK54_RS17320 - replication-associated recombination protein A, at 3,787,008 to 3,788,345 _RS17320 LRK54_RS17325: LRK54_RS17325 - hypothetical protein, at 3,788,423 to 3,789,001 _RS17325 LRK54_RS17330: LRK54_RS17330 - serine--tRNA ligase, at 3,789,142 to 3,790,422 _RS17330 LRK54_RS17335: LRK54_RS17335 - hypothetical protein, at 3,790,419 to 3,790,778 _RS17335 LRK54_RS17340: LRK54_RS17340 - tRNA-Ser, at 3,790,948 to 3,791,037 _RS17340
Group Condition LRK54_RS17320 LRK54_RS17325 LRK54_RS17330 LRK54_RS17335 LRK54_RS17340
phage 2E-T1 -0.2 -0.6 N.D. -0.1 N.D.
biofilm assay; biofilm sample biofilm; Day1; NLDM; Manganese (II) chloride tetrahydrate 10mM +0.1 -0.3 N.D. -0.6 N.D.
no phage control No phage control -0.2 -0.2 N.D. -0.3 N.D.
carbon source a-Cyclodextrin 20 mM -0.1 -0.2 N.D. -0.5 N.D.
pH R2A_PIPES pH 4.25 +0.0 -0.2 N.D. -0.5 N.D.
phage 2E-T2 -0.2 -0.3 N.D. -0.2 N.D.
phage 4E-T2 -0.3 +0.1 N.D. -0.4 N.D.
biofilm assay; planktonic sample Planktonic; Day4; SGW; pH7 +0.1 -0.4 N.D. -0.2 N.D.
phage phage 31.1 +0.2 -0.3 N.D. -0.3 N.D.
stress R2A_PIPES with Manganese (II) chloride tetrahydrate 1 mM +0.1 +0.1 N.D. -0.6 N.D.
stress R2A_PIPES with Metal_ion_mix_sitestressplate 2 x +0.1 +0.2 N.D. -0.7 N.D.
phage 2F-T2 +0.2 -0.0 N.D. -0.5 N.D.
no stress control no stress control -0.1 +0.3 N.D. -0.5 N.D.
carbon source D-Glucose (C) and 20 AA mix; 30C -0.3 -0.3 N.D. +0.3 N.D.
pH R2A_PIPES pH 4.25 -0.0 +0.3 N.D. -0.5 N.D.
phage 3E-T1 +0.1 -0.4 N.D. +0.3 N.D.
stress R2A with nitrite 50 mM -0.2 -0.2 N.D. +0.5 N.D.
stress R2A with nitrite 25 mM -0.1 +0.3 N.D. +0.3 N.D.
phage 2A-T1 -0.2 +0.2 N.D. +0.4 N.D.
anaerobic Anaerobic, acetate donor; nitrate and iron electron acceptors -0.2 +0.4 N.D. +0.3 N.D.
biofilm assay; planktonic sample planktonic; Day2; NLDM; Aluminum chloride 1mM -0.1 +0.2 N.D. +0.4 N.D.
stress R2A with lithium chloride 15.625 mM +0.1 +0.2 N.D. +0.4 N.D.
stress R2A with Nickel (II) chloride 0.3125 mM +0.2 +0.2 N.D. +0.3 N.D.
biofilm assay; planktonic sample planktonic; Day2; NLDM; Manganese (II) chloride tetrahydrate 10mM +0.1 +0.2 N.D. +0.3 N.D.
biofilm assay; biofilm sample biofilm; Day1; NLDM; Manganese (II) chloride tetrahydrate 10mM +0.4 +0.1 N.D. +0.2 N.D.
r2a_control R2A control +0.1 +0.3 N.D. +0.3 N.D.
stress R2A with lithium chloride 15.625 mM +0.1 +0.4 N.D. +0.2 N.D.
biofilm assay; biofilm sample biofilm; Day1; NLDM; +0.3 +0.3 N.D. +0.1 N.D.
biofilm assay; biofilm sample biofilm; Day2; NLDM; Manganese (II) chloride tetrahydrate 10mM +0.4 +0.2 N.D. +0.4 N.D.
biofilm assay; biofilm sample biofilm; Day2; NLDM; Aluminum chloride 1mM -0.0 +0.4 N.D. +0.6 N.D.
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