Fitness for 5 genes in Herbaspirillum seropedicae SmR1

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500 ntHSERO_RS20715 and HSERO_RS20720 are separated by 4 nucleotidesHSERO_RS20720 and HSERO_RS20725 overlap by 4 nucleotidesHSERO_RS20725 and HSERO_RS20730 are separated by 21 nucleotidesHSERO_RS20730 and HSERO_RS20735 are separated by 582 nucleotides HSERO_RS20715: HSERO_RS20715 - cell division protein FtsY, at 4,747,817 to 4,748,782 _RS20715 HSERO_RS20720: HSERO_RS20720 - GMP synthase, at 4,748,787 to 4,749,479 _RS20720 HSERO_RS20725: HSERO_RS20725 - permease, at 4,749,476 to 4,750,399 _RS20725 HSERO_RS20730: HSERO_RS20730 - hypothetical protein, at 4,750,421 to 4,751,362 _RS20730 HSERO_RS20735: HSERO_RS20735 - RNA polymerase sigma 70, at 4,751,945 to 4,752,844 _RS20735
Group Condition HSERO_RS20715 HSERO_RS20720 HSERO_RS20725 HSERO_RS20730 HSERO_RS20735
stress Cisplatin 0.003 mg/ml N.D. -2.4 -2.3 +0.0 N.D.
stress Cobalt chloride 0.2 mM N.D. -1.4 -1.7 +0.2 N.D.
stress Lomefloxacin 0.012 mM N.D. -1.1 -1.5 -0.3 N.D.
stress Sisomicin 0.125 mg/ml N.D. -1.1 -1.4 +0.1 N.D.
stress Nickel (II) chloride 0.6 mM N.D. -1.5 -0.9 +0.2 N.D.
stress nitrate 200 mM N.D. -0.7 -0.9 +0.6 N.D.
carbon source D-Galacturonic Acid (C) N.D. -0.7 +0.1 -0.1 N.D.
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days N.D. +0.4 +0.3 -0.5 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant N.D. +0.7 +0.1 -0.3 N.D.
carbon source L-Fucose (C) N.D. +0.7 +0.1 -0.3 N.D.
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days N.D. +0.5 +0.4 -0.3 N.D.
nitrogen source nitrate (N); low aeration N.D. +0.4 +0.4 -0.2 N.D.
nitrogen fixation D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 8 days N.D. +0.6 +0.3 -0.2 N.D.
carbon source L-Rhamnose (C) N.D. +0.7 +0.1 +0.2 N.D.
carbon source Glycerol (C) N.D. +0.5 +0.1 +0.4 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. +0.8 -0.0 +0.2 N.D.
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days N.D. +0.6 +0.5 -0.1 N.D.
carbon source D-Glucose (C) N.D. +0.6 +0.2 +0.3 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. +0.5 +0.4 +0.1 N.D.
carbon source D-Ribose (C) N.D. +0.7 +0.3 +0.1 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. +0.7 -0.0 +0.5 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. +0.4 +0.4 +0.4 N.D.
plant Plant assay with Setaria viridis; 10 days of inoculation with plant; outgrowth in LB N.D. +0.5 +0.2 +0.6 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. +0.1 +0.2 +0.9 N.D.
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days N.D. +0.8 +0.6 -0.1 N.D.
carbon source L-Proline (C) N.D. +0.6 +0.4 +0.4 N.D.
agar plate interaction control Taped volatile agar plate with no fungus N.D. +0.5 +0.5 +0.4 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant N.D. +0.5 +0.7 +0.2 N.D.
carbon source L-Proline (C) N.D. +0.9 +0.3 +0.2 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. +0.8 +0.4 +0.4 N.D.
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