Fitness for 5 genes in Herbaspirillum seropedicae SmR1

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500 ntHSERO_RS17780 and HSERO_RS17785 are separated by 55 nucleotidesHSERO_RS17785 and HSERO_RS17790 overlap by 4 nucleotidesHSERO_RS17790 and HSERO_RS17795 are separated by 200 nucleotidesHSERO_RS17795 and HSERO_RS17800 are separated by 46 nucleotides HSERO_RS17780: HSERO_RS17780 - 50S rRNA methyltransferase, at 4,084,711 to 4,085,181 _RS17780 HSERO_RS17785: HSERO_RS17785 - septum formation inhibitor Maf, at 4,085,237 to 4,085,851 _RS17785 HSERO_RS17790: HSERO_RS17790 - ribonuclease, at 4,085,848 to 4,087,311 _RS17790 HSERO_RS17795: HSERO_RS17795 - ligase, at 4,087,512 to 4,088,738 _RS17795 HSERO_RS17800: HSERO_RS17800 - ATP-binding protein, at 4,088,785 to 4,090,521 _RS17800
Group Condition HSERO_RS17780 HSERO_RS17785 HSERO_RS17790 HSERO_RS17795 HSERO_RS17800
plant Plant assay with Setaria viridis; 10 days of inoculation with plant; outgrowth in LB -0.3 -0.6 N.D. -1.0 N.D.
agar plate interaction Taped volatile agar plate with Trichoderma atroviridae IMI -0.6 -0.1 N.D. -0.8 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.5X; growth supplemented with 0.4X LB -0.7 -0.2 N.D. -0.4 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.5 -0.1 N.D. -0.6 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.4 -0.3 N.D. -0.5 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB -0.3 -0.2 N.D. -0.6 N.D.
supernatant control Vogels_fungal_media; pH 5.5 -0.6 -0.1 N.D. -0.4 N.D.
plant Control soil assay; outgrowth in LB -0.6 -0.1 N.D. -0.4 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.6 +0.2 N.D. -0.4 N.D.
agar plate interaction control Taped volatile agar plate with no fungus -0.6 +0.2 N.D. -0.4 N.D.
carbon source D-Glucose-6-Phosphate (C) +0.3 -0.6 N.D. -0.3 N.D.
stress nitrate 200 mM -0.2 +0.3 N.D. -0.6 N.D.
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB -0.5 +0.4 N.D. -0.3 N.D.
stress Cisplatin 0.003 mg/ml +0.0 -0.7 N.D. +0.3 N.D.
carbon source D-Gluconic Acid (C) +0.5 -0.3 N.D. -0.4 N.D.
carbon source L-Rhamnose (C) +0.4 +0.1 N.D. -0.5 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.1 +0.5 N.D. -0.5 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X +0.2 +0.6 N.D. -0.5 N.D.
carbon source D-Gluconic Acid (C) +0.7 -0.3 N.D. -0.1 N.D.
stress Sisomicin 0.125 mg/ml +0.7 -0.3 N.D. +0.1 N.D.
carbon source Glycerol (C) +0.7 -0.3 N.D. +0.2 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +1.0 -0.0 N.D. -0.3 N.D.
control NoPredeControl +0.6 -0.5 N.D. +0.7 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.1 -0.1 N.D. +0.8 N.D.
carbon source D-Glucose (C) +0.5 -0.4 N.D. +0.9 N.D.
stress Thallium(I) acetate 0.001 mg/ml +0.4 +0.2 N.D. +0.5 N.D.
vitamin With vitamins t2 +0.3 +0.5 N.D. +0.2 N.D.
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X +0.1 +0.2 N.D. +0.7 N.D.
carbon source L-Fucose (C) +0.7 -0.0 N.D. +0.5 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +1.5 +0.1 N.D. +1.2 N.D.
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