Fitness for 5 genes in Herbaspirillum seropedicae SmR1

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500 ntHSERO_RS12225 and HSERO_RS12230 are separated by 53 nucleotidesHSERO_RS12230 and HSERO_RS12235 are separated by 106 nucleotidesHSERO_RS12235 and HSERO_RS12240 overlap by 1 nucleotidesHSERO_RS12240 and HSERO_RS12245 overlap by 8 nucleotides HSERO_RS12225: HSERO_RS12225 - AraC family transcriptional regulator, at 2,776,386 to 2,777,300 _RS12225 HSERO_RS12230: HSERO_RS12230 - transcriptional regulator, at 2,777,354 to 2,777,995 _RS12230 HSERO_RS12235: HSERO_RS12235 - biotin synthase, at 2,778,102 to 2,779,145 _RS12235 HSERO_RS12240: HSERO_RS12240 - cytochrome P450, at 2,779,145 to 2,780,320 _RS12240 HSERO_RS12245: HSERO_RS12245 - 8-amino-7-oxononanoate synthase, at 2,780,313 to 2,781,470 _RS12245
Group Condition HSERO_RS12225 HSERO_RS12230 HSERO_RS12235 HSERO_RS12240 HSERO_RS12245
vitamin No vitamins t2 +0.1 N.D. -3.9 -1.5 -2.2
plant Plant assay with Setaria viridis; 10 days of inoculation with plant; outgrowth in LB -0.1 N.D. -0.3 -0.5 -0.5
plant Control soil assay; outgrowth in LB -0.1 N.D. -0.1 -0.8 -0.2
vitamin No vitamins t1 +0.3 N.D. -0.5 -0.4 -0.6
micoeukaryotes Dictyostellum sp.mixed culture-1 -0.4 N.D. -0.3 -0.4 +0.2
supernatant control Vogels_fungal_media; pH 5.5 -0.1 N.D. -0.2 -0.7 +0.3
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB -0.3 N.D. -0.3 -0.3 +0.3
stress sodium fluoride 100 mM +0.1 N.D. +0.4 -0.7 -0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.2 N.D. -0.3 +0.5 -0.5
carbon source D-Galacturonic Acid (C) +0.2 N.D. +0.4 -0.1 -0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.0 N.D. -0.4 -0.3 +0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.6 N.D. +0.2 -0.6 -0.0
stress nitrite 20 mM +0.6 N.D. -0.0 +0.1 -0.5
stress Sisomicin 0.125 mg/ml -0.0 N.D. -0.5 +0.8 +0.0
agar plate interaction control Taped volatile agar plate with no fungus +0.2 N.D. -0.0 -0.4 +0.7
micoeukaryotes Acanthamoeba sp., muxed culture-1 +0.0 N.D. -0.2 +0.8 -0.3
carbon source L-Arabinose (C) +0.1 N.D. +0.5 -0.3 +0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.5 N.D. +0.2 -0.2 +0.3
stress Lomefloxacin 0.012 mM +0.6 N.D. -0.6 +0.5 +0.4
carbon source Carbon source 2-Deoxy-D-Ribose 5 mM -0.1 N.D. +0.2 -0.1 +1.0
carbon source D-Galacturonic Acid (C) +0.3 N.D. +0.3 +0.5 -0.1
carbon source a-Ketoglutaric (C) +0.3 N.D. +0.4 +0.4 -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.1 N.D. +0.3 +0.2 +0.8
carbon source D-Xylose (C) +0.2 N.D. +0.4 +0.3 +0.4
carbon source Xylitol (C) +0.2 N.D. +0.3 +0.3 +0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.5 N.D. +0.1 +0.4 +0.3
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.0 N.D. +0.3 +0.4 +0.6
carbon source L-Fucose (C) +0.5 N.D. +0.6 +0.2 +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.0 N.D. +0.1 -0.1 +1.4
carbon source D-Gluconic Acid (C) +0.4 N.D. +0.2 +0.6 +0.3
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