Fitness for 5 genes in Herbaspirillum seropedicae SmR1

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500 ntHSERO_RS10615 and HSERO_RS10620 are separated by 124 nucleotidesHSERO_RS10620 and HSERO_RS10625 are separated by 101 nucleotidesHSERO_RS10625 and HSERO_RS10630 are separated by 200 nucleotidesHSERO_RS10630 and HSERO_RS10635 are separated by 109 nucleotides HSERO_RS10615: HSERO_RS10615 - transcription elongation factor GreB, at 2,389,529 to 2,390,086 _RS10615 HSERO_RS10620: HSERO_RS10620 - membrane protein, at 2,390,211 to 2,391,551 _RS10620 HSERO_RS10625: HSERO_RS10625 - FMN-dependent NADH-azoreductase, at 2,391,653 to 2,392,261 _RS10625 HSERO_RS10630: HSERO_RS10630 - LysR family transcriptional regulator, at 2,392,462 to 2,393,436 _RS10630 HSERO_RS10635: HSERO_RS10635 - guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase, at 2,393,546 to 2,395,807 _RS10635
Group Condition HSERO_RS10615 HSERO_RS10620 HSERO_RS10625 HSERO_RS10630 HSERO_RS10635
carbon source L-Proline (C) -0.6 -1.4 -0.4 -0.2 N.D.
carbon source L-Proline (C) -0.5 -1.3 -0.0 +0.1 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X +0.0 -0.1 -0.5 -0.9 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X -0.2 -0.5 -0.4 -0.4 N.D.
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB -0.2 -0.2 -0.8 -0.4 N.D.
vitamin No vitamins t1 +0.2 -0.5 -0.4 -0.5 N.D.
stress Cisplatin 0.003 mg/ml -0.4 +0.1 -0.9 +0.1 N.D.
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days -1.5 +0.1 +0.2 +0.1 N.D.
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days -1.5 +0.4 +0.1 -0.1 N.D.
carbon source D-Lactate (C) +0.3 -1.1 +0.3 -0.2 N.D.
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days -1.1 +0.2 +0.2 +0.1 N.D.
agar plate interaction control Taped volatile agar plate with no fungus +0.5 +0.1 -0.5 -0.7 N.D.
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days -1.2 +0.3 +0.1 +0.3 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.1 +0.5 -0.6 -0.6 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.2 -0.6 +0.3 -0.4 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.6 -0.3 +0.1 -0.6 N.D.
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days -0.8 +0.2 +0.2 +0.3 N.D.
carbon source D-Galactose (C) +0.4 -0.2 +0.5 -0.5 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.3 -0.8 +0.3 +0.4 N.D.
nitrogen source L-Proline (N) +0.3 +0.6 -0.3 -0.2 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.4 -0.4 -0.1 +0.5 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.2 +1.3 -0.6 -0.4 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.3 +0.8 -0.1 -0.3 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.1 +0.0 +1.1 -0.4 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.6 +0.1 +0.6 -0.3 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.9 +0.1 +0.3 -0.3 N.D.
plant Plant assay with Setaria viridis; 10 days of inoculation with plant; outgrowth in LB -0.3 -0.1 +1.2 +0.5 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +1.2 +0.4 +0.3 -0.4 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.0 +0.3 -0.1 +1.3 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +1.1 +0.0 +0.6 -0.2 N.D.
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