Fitness for 5 genes in Herbaspirillum seropedicae SmR1

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500 ntHSERO_RS08500 and HSERO_RS08505 are separated by 307 nucleotidesHSERO_RS08505 and HSERO_RS08510 are separated by 34 nucleotidesHSERO_RS08510 and HSERO_RS08515 are separated by 23 nucleotidesHSERO_RS08515 and HSERO_RS08520 are separated by 824 nucleotides HSERO_RS08500: HSERO_RS08500 - hypothetical protein, at 1,934,078 to 1,935,895 _RS08500 HSERO_RS08505: HSERO_RS08505 - methyl-accepting chemotaxis protein, at 1,936,203 to 1,937,771 _RS08505 HSERO_RS08510: HSERO_RS08510 - phosphodiesterase, at 1,937,806 to 1,939,572 _RS08510 HSERO_RS08515: HSERO_RS08515 - diguanylate cyclase, at 1,939,596 to 1,940,348 _RS08515 HSERO_RS08520: HSERO_RS08520 - major facilitator transporter, at 1,941,173 to 1,942,498 _RS08520
Group Condition HSERO_RS08500 HSERO_RS08505 HSERO_RS08510 HSERO_RS08515 HSERO_RS08520
micoeukaryotes C.elegans, mixed culture-1 +0.3 -0.4 +0.2 -5.4 +0.2
micoeukaryotes C.elegans, mixed culture-2 +0.0 -0.1 -0.3 -3.8 -0.1
micoeukaryotes Acanthamoeba sp., muxed culture-1 +0.0 +0.0 +0.3 -4.1 -0.4
nitrogen fixation D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 8 days +0.1 +0.1 -0.1 -3.0 -0.0
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days -0.1 +0.1 +0.1 -2.9 +0.2
carbon source L-Rhamnose (C) -0.3 -0.2 -0.0 -1.8 -0.2
agar plate interaction Taped volatile agar plate with Trichoderma atroviridae IMI +0.1 +0.1 +0.1 -2.6 -0.2
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days +0.0 -0.0 -0.0 -2.4 +0.1
micoeukaryotes Acanthamoeba sp., muxed culture-2 +0.1 -0.1 +0.0 -2.8 +0.4
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days +0.1 +0.1 -0.1 -2.4 +0.1
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB -0.3 -0.5 -0.1 -1.7 +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.1 +0.2 -0.1 -2.1 -0.3
carbon source D-Mannose (C) +0.3 -0.1 +0.1 -1.8 -0.5
agar plate interaction control Taped volatile agar plate with no fungus -0.2 -0.2 +0.3 -1.7 -0.2
supernatant control Vogels_fungal_media 0.5X; growth supplemented with 0.4X LB -0.3 +0.2 -0.1 -1.8 +0.0
stress Cobalt chloride 0.2 mM +0.4 -0.2 -0.1 -1.5 -0.6
vitamin With vitamins t2 +0.1 +0.1 +0.2 -2.1 -0.0
plant Plant assay with Setaria viridis; 10 days of inoculation with plant; outgrowth in LB -0.3 -0.1 +0.3 -1.6 -0.1
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days +0.1 +0.1 -0.2 -1.9 +0.2
stress nitrite 20 mM -0.3 +0.0 +0.3 -1.7 +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.2 +0.2 -0.0 -2.1 +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.6 -0.1 -0.4 -1.0 -0.4
carbon source D-Galactose (C) +0.4 +0.3 +0.3 -1.9 -0.4
carbon source L-Fucose (C) -0.3 +0.0 +0.3 -1.4 +0.3
carbon source D-Galactose (C) +0.0 +0.3 +0.4 -1.1 -0.6
supernatant control Vogels_fungal_media; pH 5.5 -0.1 +0.5 +0.2 -1.4 -0.2
plant Plant assay with Setaria viridis; 10 days of inoculation with plant; outgrowth in LB -0.3 -0.1 +0.4 -1.7 +0.8
carbon source L-Asparagine (C) +0.3 +0.1 +0.3 -1.5 +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.2 -0.0 +0.1 -1.3 +1.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.3 -0.1 -0.3 +2.0 -0.6
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