Fitness for 5 genes in Herbaspirillum seropedicae SmR1

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500 ntHSERO_RS06140 and HSERO_RS06145 are separated by 274 nucleotidesHSERO_RS06145 and HSERO_RS06150 are separated by 467 nucleotidesHSERO_RS06150 and HSERO_RS06155 overlap by 4 nucleotidesHSERO_RS06155 and HSERO_RS06160 are separated by 178 nucleotides HSERO_RS06140: HSERO_RS06140 - serine hydroxymethyltransferase, at 1,381,528 to 1,382,772 _RS06140 HSERO_RS06145: HSERO_RS06145 - NrdR family transcriptional regulator, at 1,383,047 to 1,383,526 _RS06145 HSERO_RS06150: HSERO_RS06150 - cell division protein ZapA, at 1,383,994 to 1,384,311 _RS06150 HSERO_RS06155: HSERO_RS06155 - BRLZ domain containing protein, at 1,384,308 to 1,384,538 _RS06155 HSERO_RS06160: HSERO_RS06160 - hypothetical protein, at 1,384,717 to 1,385,412 _RS06160
Group Condition HSERO_RS06140 HSERO_RS06145 HSERO_RS06150 HSERO_RS06155 HSERO_RS06160
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant N.D. -3.5 -0.2 N.D. +0.1
supernatant control Vogels_fungal_media; pH 5.5 N.D. -3.4 +0.2 N.D. -0.4
carbon source L-Fucose (C) N.D. -3.2 -0.1 N.D. -0.0
carbon source D-Ribose (C) N.D. -3.1 -0.4 N.D. +0.2
plant Plant assay with Setaria viridis; 10 days of inoculation with plant; outgrowth in LB N.D. -2.4 -0.1 N.D. -0.7
carbon source D-Mannose (C) N.D. -3.5 +0.3 N.D. +0.1
stress nitrate 200 mM N.D. -0.9 -2.0 N.D. -0.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB N.D. -3.3 -0.1 N.D. +0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -2.5 -0.3 N.D. +0.1
stress Cisplatin 0.003 mg/ml N.D. +0.7 -2.9 N.D. -0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant N.D. -1.8 -0.3 N.D. -0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant N.D. -2.1 -0.4 N.D. -0.1
carbon source D-Xylose (C) N.D. -2.3 -0.0 N.D. -0.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. -2.4 +0.2 N.D. -0.1
supernatant control:fungal media R2A with Vogels_fungal_media 0.8X N.D. -2.3 +0.2 N.D. -0.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -2.0 +0.3 N.D. -0.2
r2a control R2A control N.D. -1.8 +0.1 N.D. -0.2
carbon source L-Arabinose (C) N.D. -2.1 +0.0 N.D. +0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -1.9 +0.6 N.D. -0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -2.4 +0.4 N.D. +0.2
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB N.D. -1.8 +0.2 N.D. -0.2
control NoPredeControl N.D. -2.5 +0.5 N.D. +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. -2.0 +0.1 N.D. +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -2.2 +0.9 N.D. -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -2.0 +0.4 N.D. +0.1
nitrogen fixation D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 8 days N.D. -1.9 +0.2 N.D. +0.2
supernatant control Vogels_fungal_media 0.5X; growth supplemented with 0.4X LB N.D. -1.8 -0.0 N.D. +0.3
stress Nickel (II) chloride 0.6 mM N.D. +0.8 -1.3 N.D. +0.0
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -1.2 +0.4 N.D. +0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant N.D. -1.0 +1.0 N.D. -0.2
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