Fitness for 5 genes in Herbaspirillum seropedicae SmR1

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500 ntHSERO_RS05900 and HSERO_RS05905 are separated by 122 nucleotidesHSERO_RS05905 and HSERO_RS05910 are separated by 72 nucleotidesHSERO_RS05910 and HSERO_RS05915 are separated by 51 nucleotidesHSERO_RS05915 and HSERO_RS05920 overlap by 4 nucleotides HSERO_RS05900: HSERO_RS05900 - ribonuclease, at 1,342,022 to 1,342,417 _RS05900 HSERO_RS05905: HSERO_RS05905 - barnase inhibitor, at 1,342,540 to 1,342,938 _RS05905 HSERO_RS05910: HSERO_RS05910 - phosphoribosylglycinamide formyltransferase, at 1,343,011 to 1,343,673 _RS05910 HSERO_RS05915: HSERO_RS05915 - SAM-dependent methyltransferase, at 1,343,725 to 1,344,984 _RS05915 HSERO_RS05920: HSERO_RS05920 - endonuclease, at 1,344,981 to 1,345,565 _RS05920
Group Condition HSERO_RS05900 HSERO_RS05905 HSERO_RS05910 HSERO_RS05915 HSERO_RS05920
carbon source L-Malic (C) -0.8 N.D. -3.4 -1.6 -2.5
control NoPredeControl +0.1 N.D. -3.8 -0.5 -1.8
carbon source L-Malic (C) -0.3 N.D. -1.3 -1.6 -2.7
nitrogen fixation control D-Glucose (C) with ammonium chloride; anaerobic; 3 days -1.1 N.D. -2.6 -0.3 -0.8
vitamin With vitamins t2 -0.6 N.D. -3.0 +0.0 -0.4
carbon source D-Glucose (C) -0.5 N.D. -2.0 -0.3 -1.2
nitrogen source nitrate (N); high aeration -0.3 N.D. -3.6 +0.0 +0.0
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB -0.6 N.D. -2.6 +0.2 -0.7
vitamin No vitamins t2 -0.7 N.D. -2.9 +0.3 -0.4
carbon source succinate (C) -0.1 N.D. -1.9 -0.4 -1.1
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.1 N.D. -3.9 +0.4 +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.3 N.D. -0.7 -0.2 -2.2
nitrogen fixation control D-Glucose (C) with ammonium chloride; anaerobic; 8 days -0.5 N.D. -2.4 +0.2 -0.5
nitrogen fixation control D-Glucose (C) with ammonium chloride; anaerobic; 8 days -0.9 N.D. -2.1 +0.2 -0.3
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB -0.5 N.D. -3.0 +0.3 +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.6 N.D. -1.7 -0.8 -0.0
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.5 N.D. -1.7 -0.5 -0.4
vitamin No vitamins t1 -0.3 N.D. -2.5 +0.2 -0.4
carbon source L-Glutamine (C) -0.1 N.D. -2.2 -0.3 -0.5
micoeukaryotes Dictyostellum sp.mixed culture-2 -0.2 N.D. -2.1 +0.1 -0.8
nitrogen source nitrate (N); low aeration -0.2 N.D. -2.7 -0.0 +0.1
carbon source L-Glutamine (C) +0.1 N.D. -2.7 -0.4 +0.2
carbon source a-Ketoglutaric (C) +0.3 N.D. -1.4 -0.6 -1.0
carbon source succinate (C) +0.5 N.D. -1.4 -1.0 -0.6
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days -0.3 N.D. -2.5 +0.6 +0.1
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days -0.2 N.D. -2.3 +0.5 -0.0
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days +0.4 N.D. -2.6 +0.5 -0.1
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days +0.1 N.D. -1.9 +0.8 -0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.3 N.D. -2.1 -0.2 +1.3
plant Plant assay with Setaria viridis; 10 days of inoculation with plant; outgrowth in LB +0.2 N.D. +3.3 -0.3 -0.2
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