Fitness for 5 genes in Herbaspirillum seropedicae SmR1

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500 ntHSERO_RS05010 and HSERO_RS05015 are separated by 32 nucleotidesHSERO_RS05015 and HSERO_RS05020 are separated by 97 nucleotidesHSERO_RS05020 and HSERO_RS05025 are separated by 342 nucleotidesHSERO_RS05025 and HSERO_RS05030 are separated by 30 nucleotides HSERO_RS05010: HSERO_RS05010 - filamentous hemagglutinin outer membrane protein, at 1,103,337 to 1,108,043 _RS05010 HSERO_RS05015: HSERO_RS05015 - hemin transporter, at 1,108,076 to 1,109,749 _RS05015 HSERO_RS05020: HSERO_RS05020 - transcriptional regulator, at 1,109,847 to 1,110,776 _RS05020 HSERO_RS05025: HSERO_RS05025 - hypothetical protein, at 1,111,119 to 1,111,982 _RS05025 HSERO_RS05030: HSERO_RS05030 - hydrolase, at 1,112,013 to 1,113,173 _RS05030
Group Condition HSERO_RS05010 HSERO_RS05015 HSERO_RS05020 HSERO_RS05025 HSERO_RS05030
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB +0.1 -0.1 -0.7 -0.2 -0.4
vitamin With vitamins t2 -0.1 -0.1 -0.8 -0.2 -0.1
micoeukaryotes Acanthamoeba sp., muxed culture-1 -0.0 -0.3 +0.1 -0.6 -0.3
plant Plant assay with Setaria viridis; 10 days of inoculation with plant; outgrowth in LB -0.0 +0.1 -0.4 -0.8 +0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.0 -0.3 -0.4 +0.6 -0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.1 -0.2 +0.4 -0.7 +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.1 +0.1 +0.3 -0.7 -0.1
stress Thallium(I) acetate 0.001 mg/ml -0.1 -0.5 +0.3 +0.2 -0.1
agar plate interaction Taped volatile agar plate with Trichoderma atroviridae IMI -0.2 -0.4 +0.0 +0.8 -0.3
nitrogen fixation control D-Glucose (C) with ammonium chloride; anaerobic; 8 days -0.4 -0.4 +0.4 +0.4 +0.0
vitamin No vitamins t1 -0.1 +0.2 -0.4 +0.4 -0.2
carbon source L-Rhamnose (C) -0.0 -0.1 +0.3 +0.4 -0.4
carbon source D-Galactose (C) -0.0 -0.0 +0.2 +0.6 -0.4
supernatant control Vogels_fungal_media; pH 5.5 -0.1 -0.2 -0.1 +0.8 +0.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.3 +0.2 -0.4 +0.1 +0.2
stress Lomefloxacin 0.012 mM -0.3 -0.2 +0.3 +0.8 -0.1
carbon source D-Lactate (C) +0.0 +0.3 +0.4 +0.3 -0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.0 +0.1 -0.3 +0.8 +0.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.1 +0.0 +0.9 -0.4 +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.0 +0.9 -0.5 +0.3 +0.0
stress A22 0.04 mg/ml +0.0 -0.3 +0.5 +0.3 +0.2
carbon source L-Fucose (C) +0.1 +0.2 +0.3 +0.4 -0.2
supernatant control:fungal media R2A with Vogels_fungal_media 0.8X -0.0 +0.1 +0.3 +0.6 -0.1
carbon source D-Gluconic Acid (C) -0.2 +0.4 +0.5 +0.3 -0.1
carbon source D-Xylose (C) -0.3 +0.2 +0.4 +0.5 -0.0
carbon source a-Ketoglutaric (C) -0.1 -0.1 +0.6 +0.7 -0.1
carbon source Glycerol (C) -0.1 -0.2 +0.5 +0.7 +0.2
carbon source D-Glucose (C) -0.1 +0.1 +0.2 +0.9 +0.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.0 +0.5 -0.1 +0.7 +0.1
plant Plant assay with Setaria viridis; 10 days of inoculation with plant; outgrowth in LB +0.2 -0.1 +0.8 +1.2 -0.1
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