Fitness for 5 genes in Herbaspirillum seropedicae SmR1

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500 ntHSERO_RS01275 and HSERO_RS01280 are separated by 134 nucleotidesHSERO_RS01280 and HSERO_RS01285 are separated by 41 nucleotidesHSERO_RS01285 and HSERO_RS01290 are separated by 102 nucleotidesHSERO_RS01290 and HSERO_RS01295 are separated by 237 nucleotides HSERO_RS01275: HSERO_RS01275 - FAD-dependent oxidoreductase, at 269,035 to 270,312 _RS01275 HSERO_RS01280: HSERO_RS01280 - methyl-accepting chemotaxis protein, at 270,447 to 272,240 _RS01280 HSERO_RS01285: HSERO_RS01285 - diguanylate cyclase, at 272,282 to 273,436 _RS01285 HSERO_RS01290: HSERO_RS01290 - hypothetical protein, at 273,539 to 273,724 _RS01290 HSERO_RS01295: HSERO_RS01295 - deoxyuridine 5'-triphosphate nucleotidohydrolase, at 273,962 to 275,932 _RS01295
Group Condition HSERO_RS01275 HSERO_RS01280 HSERO_RS01285 HSERO_RS01290 HSERO_RS01295
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.5 -0.1 +0.2 -0.9 +0.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.7 +0.2 -0.1 +0.2 -0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.6 -0.2 +0.4 -0.3 +0.1
plant Plant assay with Setaria viridis; 10 days of inoculation with plant; outgrowth in LB -0.0 +0.1 -0.1 -0.9 +0.7
micoeukaryotes C.elegans, mixed culture-2 -0.2 -0.3 -0.0 -0.5 +0.7
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB +0.1 +0.3 +0.1 -0.8 +0.1
carbon source D-Lactate (C) -0.5 -0.5 +0.2 +0.8 +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.1 +0.4 -0.4 +0.6 -0.0
carbon source D-Xylose (C) -0.7 +0.2 +0.1 +0.9 -0.1
micoeukaryotes Acanthamoeba sp., muxed culture-1 +0.5 -0.1 +0.2 -0.4 +0.5
plant Plant assay with Setaria viridis; 10 days of inoculation with plant; outgrowth in LB -0.1 +0.1 -0.3 -0.4 +1.3
vitamin With vitamins t2 +0.5 +0.0 -0.0 -0.4 +0.6
control NoPredeControl +0.1 -0.1 +0.1 -0.3 +1.0
plant Plant assay with Setaria viridis; 10 days of inoculation with plant; outgrowth in LB -0.1 -0.4 -0.2 -0.1 +1.6
vitamin No vitamins t2 +0.1 +0.3 +0.2 -0.6 +0.8
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.3 +0.2 +0.4 +0.5 +0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.1 -0.2 +0.2 +0.8 +0.3
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days +0.2 +0.1 +0.0 -1.0 +2.0
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.1 +0.1 +0.0 +1.2 +0.3
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days +0.2 +0.1 +0.0 -0.5 +2.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.4 -0.1 +0.1 +1.7 -0.1
nitrogen fixation control D-Glucose (C) with ammonium chloride; anaerobic; 3 days +0.2 +0.1 +0.2 +0.2 +1.5
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days +0.1 -0.1 +0.0 -0.4 +2.9
plant Plant assay with Setaria viridis; 10 days of inoculation with plant; outgrowth in LB +0.1 +0.6 +0.1 +0.8 +1.1
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days +0.2 +0.0 +0.3 -0.7 +3.3
nitrogen fixation D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 8 days +0.2 +0.1 +0.1 +0.0 +3.1
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days -0.0 -0.1 +0.0 -1.0 +5.2
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days +0.1 +0.3 +0.1 -1.1 +4.7
nitrogen fixation control D-Glucose (C) with ammonium chloride; anaerobic; 8 days +0.0 +0.1 +0.1 +0.1 +4.3
nitrogen fixation control D-Glucose (C) with ammonium chloride; anaerobic; 8 days +0.2 +0.1 +0.2 -0.5 +8.1
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