Fitness for 5 genes in Sphingomonas koreensis DSMZ 15582

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500 ntGa0059261_0298 and Ga0059261_0299 are separated by 85 nucleotidesGa0059261_0299 and Ga0059261_0300 are separated by 4 nucleotidesGa0059261_0300 and Ga0059261_0301 are separated by 185 nucleotidesGa0059261_0301 and Ga0059261_0302 are separated by 27 nucleotides Ga0059261_0298: Ga0059261_0298 - TonB family C-terminal domain, at 281,523 to 282,278 _0298 Ga0059261_0299: Ga0059261_0299 - phosphoribosylformylglycinamidine synthase II, at 282,364 to 284,589 _0299 Ga0059261_0300: Ga0059261_0300 - diguanylate cyclase (GGDEF) domain, at 284,594 to 286,126 _0300 Ga0059261_0301: Ga0059261_0301 - hypothetical protein, at 286,312 to 286,719 _0301 Ga0059261_0302: Ga0059261_0302 - hypothetical protein, at 286,747 to 287,184 _0302
Group Condition Ga0059261_0298 Ga0059261_0299 Ga0059261_0300 Ga0059261_0301 Ga0059261_0302
motility inner cut, LB soft agar motility assay +0.1 N.D. -0.4 +0.3 -0.8
nutrient D-Cellobiose (C) and 0.25mM L-Methionine; no added vitamins -0.5 N.D. -0.0 -0.1 -0.0
motility outer cut, LB soft agar motility assay -0.4 N.D. -0.1 -0.3 +0.3
nitrogen source L-Glutamine (N) -0.5 N.D. +0.0 -0.1 +0.3
motility inner cut, LB soft agar motility assay +0.2 N.D. +0.1 -0.4 +0.0
nutrient D-Cellobiose (C) and 0.25mM L-Methionine; no added vitamins -0.5 N.D. +0.3 +0.2 +0.1
stress 1-ethyl-3-methylimidazolium acetate 5 mM +0.2 N.D. -0.2 -0.1 +0.1
stress 1-ethyl-3-methylimidazolium chloride 10 mM +0.2 N.D. -0.3 +0.0 +0.1
nutrient D-Cellobiose (C); no added vitamins -0.3 N.D. +0.2 +0.0 +0.3
carbon source D-Maltose (C) -0.2 N.D. +0.0 -0.1 +0.3
motility inner cut, LB soft agar motility assay -0.3 N.D. +0.0 +0.4 +0.1
stress nitrite 5 mM +0.2 N.D. +0.1 +0.2 -0.2
nutrient D-Glucose (C); no added vitamins -0.2 N.D. +0.0 +0.4 +0.0
stress Tetracycline 0.002 mg/ml -0.1 N.D. +0.1 +0.3 -0.1
nutrient D-Glucose (C); no added vitamins -0.1 N.D. +0.1 -0.1 +0.4
carbon source a-Cyclodextrin (C) -0.1 N.D. -0.1 +0.2 +0.3
pH LB, pH6 +0.1 N.D. +0.2 -0.1 +0.1
stress 1-ethyl-3-methylimidazolium lysine 10 mM +0.4 N.D. -0.0 +0.1 -0.1
motility inner cut, LB soft agar motility assay -0.0 N.D. +0.0 -0.3 +0.7
stress 1-ethyl-3-methylimidazolium acetate 20 mM +0.3 N.D. +0.1 +0.0 -0.1
nutrient L-Glutamic (C); no added vitamins -0.0 N.D. +0.2 +0.1 +0.2
nutrient D-Cellobiose (C); no added vitamins -0.6 N.D. +0.2 +0.4 +0.5
carbon source D-Maltose (C) +0.0 N.D. +0.0 +0.1 +0.4
carbon source NAG (C) +0.2 N.D. -0.0 +0.1 +0.2
pH LB, pH8 +0.2 N.D. +0.2 +0.1 +0.1
carbon source D-Glucose (C) +0.3 N.D. +0.0 +0.2 +0.1
nutrient L-Glutamic (C) and 0.25mM L-Methionine; no added vitamins +0.4 N.D. +0.1 -0.0 +0.1
nitrogen source L-Alanine (N) +0.2 N.D. +0.1 +0.1 +0.2
pH LB, pH6 +0.2 N.D. +0.2 +0.0 +0.2
nutrient D-Glucose (C) and 0.25mM L-Methionine; no added vitamins +0.3 N.D. +0.3 +0.2 +0.1
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