Fitness for 5 genes in Pseudomonas stutzeri RCH2

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500 ntPsest_4013 and Psest_4014 are separated by 76 nucleotidesPsest_4014 and Psest_4015 are separated by 100 nucleotidesPsest_4015 and Psest_4016 are separated by 166 nucleotidesPsest_4016 and Psest_4017 overlap by 8 nucleotides Psest_4013: Psest_4013 - TRAP transporter, DctM subunit, at 4,190,049 to 4,191,425 _4013 Psest_4014: Psest_4014 - hypothetical protein, at 4,191,502 to 4,192,719 _4014 Psest_4015: Psest_4015 - tRNA_Thr_TGT, at 4,192,820 to 4,192,895 _4015 Psest_4016: Psest_4016 - Integrase, at 4,193,062 to 4,194,237 _4016 Psest_4017: Psest_4017 - hypothetical protein, at 4,194,230 to 4,194,979 _4017
Group Condition Psest_4013 Psest_4014 Psest_4015 Psest_4016 Psest_4017
carbon source L-Proline (C) -0.6 -0.3 N.D. -0.6 -0.5
carbon source RCH2 defined media -0.2 +0.1 N.D. -0.7 -1.0
stress Def Carbenicillin 0.008 mg/ml +0.3 +0.1 N.D. -0.7 -1.2
stress nitrate 250 mM +0.2 -0.1 N.D. -0.7 -1.0
stress Fusidic 0.016 mg/ml -0.2 +0.2 N.D. -0.6 -0.9
temperature LB 30C timecourse1 timepoint 3 -0.1 -0.3 N.D. -0.5 -0.6
metal limitation Lactate; aerobic; with copper, sample3 -0.4 -0.3 N.D. -0.2 -0.5
stress Def Carbenicillin 0.02 mg/ml -0.5 -0.3 N.D. -0.3 -0.3
metal limitation Lactate-nitrite; anaerobic; sample3 -0.1 -0.2 N.D. -0.4 -0.5
metal limitation Lactate-nitrite; anaerobic; sample2 -0.1 -0.3 N.D. -0.3 -0.5
stress Nalidixic 0.001 mg/ml -0.3 -0.2 N.D. -0.4 -0.4
carbon source L-Isoleucine (C) -1.0 -0.1 N.D. -0.1 +0.0
metal limitation Lactate-nitrite; anaerobic; sample1 -0.4 -0.4 N.D. -0.0 -0.4
metal limitation Lactate; aerobic; with copper, sample2 -0.2 -0.3 N.D. -0.3 -0.4
stress Def Polymyxin B sulfate 0.00008 mg/ml -0.0 +0.2 N.D. -0.5 -0.8
stress Gentamicin 0.0016 mg/ml +0.0 +0.2 N.D. -0.4 -1.0
stress Lactate-nitrate; anaerobic; copper stress sample3 -0.3 -0.1 N.D. -0.4 -0.4
stress Cephalothin 0.04 mg/ml +0.4 +0.4 N.D. -0.7 -1.2
carbon source L-Lactate (C) -0.0 +0.3 N.D. -0.5 -0.8
stress Choline chloride 90 mM -0.5 -0.7 N.D. +0.1 +0.0
stress Def Benzethonium chloride 0.008 mM -0.0 +0.5 N.D. -0.4 -0.9
nitrogen source nitrite (N) +0.2 +0.3 N.D. -0.5 -0.6
RCH2 grown on a colony of 8R2A +0.1 +0.2 N.D. -0.8 -0.2
RCH2 grown on a colony of Serratia -0.3 -0.7 N.D. +0.3 +0.3
stress Def Benzalkonium Chloride 0.002 mg/ml +0.2 +0.5 N.D. -0.3 -0.4
nitrogen source L-Isoleucine (N) -0.3 +0.4 N.D. +0.4 +0.1
stress Def Chlorate 20 mM +0.6 +0.2 N.D. +0.0 +0.3
lb LB +0.4 +0.1 N.D. +0.2 +0.4
stress Spectinomycin 0.00625 mg/ml +0.6 +0.6 N.D. +0.1 +0.2
stress nitrate 200 mM +0.6 +0.7 N.D. +0.3 -0.0
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