Fitness for 5 genes in Pseudomonas stutzeri RCH2

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500 ntPsest_3402 and Psest_3403 are separated by 57 nucleotidesPsest_3403 and Psest_3404 are separated by 144 nucleotidesPsest_3404 and Psest_3405 are separated by 664 nucleotidesPsest_3405 and Psest_3406 are separated by 124 nucleotides Psest_3402: Psest_3402 - PilZ domain., at 3,570,171 to 3,570,539 _3402 Psest_3403: Psest_3403 - Putative protein-S-isoprenylcysteine methyltransferase, at 3,570,597 to 3,571,058 _3403 Psest_3404: Psest_3404 - Glycine/serine hydroxymethyltransferase, at 3,571,203 to 3,572,456 _3404 Psest_3405: Psest_3405 - Type I restriction-modification system methyltransferase subunit, at 3,573,121 to 3,575,097 _3405 Psest_3406: Psest_3406 - Restriction endonuclease S subunits, at 3,575,222 to 3,576,550 _3406
Group Condition Psest_3402 Psest_3403 Psest_3404 Psest_3405 Psest_3406
carbon source L-Proline (C) N.D. -0.2 N.D. -0.7 -1.0
temperature LB 30C timecourse1 timepoint 3 N.D. -0.6 N.D. -0.6 -0.6
carbon source L-Proline (C) N.D. -0.0 N.D. -0.4 -1.0
stress Def Carbenicillin 0.008 mg/ml N.D. -0.1 N.D. -0.4 -0.9
carbon source L-Proline (C) N.D. -0.0 N.D. -0.4 -0.8
stress Def Carbenicillin 0.02 mg/ml N.D. -0.5 N.D. -0.4 -0.3
stress Polymyxin B sulfate 0.0001 mg/ml N.D. -0.7 N.D. -0.2 -0.2
RCH2 grown on a colony of N2F3 N.D. -0.7 N.D. -0.4 -0.1
stress Cephalothin 0.04 mg/ml N.D. -0.1 N.D. -0.1 -0.8
stress Gentamicin 0.0016 mg/ml N.D. -0.1 N.D. -0.2 -0.6
temperature LB 30C timecourse2 timepoint 1 N.D. -0.3 N.D. -0.3 -0.3
temperature LB 30C timecourse1 timepoint 2 N.D. -0.3 N.D. -0.3 -0.3
carbon source L-tyrosine (C) N.D. -0.0 N.D. -0.0 -0.8
stress A22 0.002 mg/ml N.D. -0.3 N.D. -0.2 -0.3
stress L-Lysine 200 mM N.D. -0.3 N.D. -0.0 -0.4
carbon source L-tyrosine (C) N.D. +0.4 N.D. -0.3 -0.7
stress Choline Lysine 20 mM N.D. -0.6 N.D. +0.1 +0.3
stress Doxycycline hyclate 0.0002 mg/ml N.D. -0.6 N.D. +0.1 +0.2
stress Def Benzethonium chloride 0.008 mM N.D. +0.2 N.D. +0.2 -0.4
stress Choline chloride 90 mM N.D. +0.4 N.D. -0.2 -0.2
stress Nalidixic 0.001 mg/ml N.D. -0.6 N.D. +0.3 +0.5
RCH2 grown on a colony of Pseudomonas migulae strain N1B4 N.D. +0.6 N.D. -0.1 -0.3
nitrogen source L-Proline (N) N.D. -0.2 N.D. +0.3 +0.3
RCH2 grown on a colony of ecoli N.D. +0.3 N.D. -0.2 +0.3
stress Paraquat dichloride 0.015 mg/ml N.D. +0.7 N.D. -0.1 -0.0
stress Def Benzalkonium Chloride 0.002 mg/ml N.D. +0.4 N.D. +0.5 -0.1
lb LB N.D. +0.3 N.D. +0.3 +0.3
stress Def Chlorate 20 mM N.D. +0.2 N.D. +0.4 +0.3
stress Phosphomycin 0.012 mg/ml N.D. +0.4 N.D. +0.2 +0.4
lb LB N.D. +0.3 N.D. +0.4 +0.5
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