Fitness for 5 genes in Pseudomonas stutzeri RCH2

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500 ntPsest_2580 and Psest_2581 are separated by 98 nucleotidesPsest_2581 and Psest_2582 overlap by 17 nucleotidesPsest_2582 and Psest_2583 overlap by 4 nucleotidesPsest_2583 and Psest_2584 are separated by 210 nucleotides Psest_2580: Psest_2580 - Uncharacterized membrane protein, required for colicin V production, at 2,760,778 to 2,761,323 _2580 Psest_2581: Psest_2581 - Uncharacterized protein conserved in bacteria, at 2,761,422 to 2,762,054 _2581 Psest_2582: Psest_2582 - folylpolyglutamate synthase/dihydrofolate synthase, at 2,762,038 to 2,763,342 _2582 Psest_2583: Psest_2583 - acetyl-CoA carboxylase, carboxyl transferase, beta subunit, at 2,763,339 to 2,764,232 _2583 Psest_2584: Psest_2584 - Phosphoribosylanthranilate isomerase, at 2,764,443 to 2,765,066 _2584
Group Condition Psest_2580 Psest_2581 Psest_2582 Psest_2583 Psest_2584
stress Def Benzethonium chloride 0.008 mM -1.5 -2.0 N.D. N.D. -6.5
stress nitrate 200 mM -0.9 -3.1 N.D. N.D. -5.6
stress Def Carbenicillin 0.008 mg/ml -1.5 -4.3 N.D. N.D. -2.4
stress Def methylglyoxal 0.01 vol% -0.3 -0.9 N.D. N.D. -6.9
stress Gentamicin 0.0016 mg/ml -1.8 -1.3 N.D. N.D. -4.8
carbon source L-Proline (C) -0.2 -0.4 N.D. N.D. -7.2
stress Def Polymyxin B sulfate 0.00008 mg/ml -1.5 -0.6 N.D. N.D. -5.6
stress Def Cephalothin 0.04 mg/ml -0.9 -1.0 N.D. N.D. -5.7
nitrogen source L-Glutamic (N) -0.5 -1.2 N.D. N.D. -6.0
carbon source RCH2 defined media -1.5 -0.6 N.D. N.D. -5.3
carbon source L-Leucine (C) -0.1 -0.5 N.D. N.D. -6.8
temperature Glucose defined 30C timecourse1 timepoint 3 -0.7 -0.4 N.D. N.D. -6.3
nitrogen source D-Alanine (N) -0.3 -0.7 N.D. N.D. -6.2
stress Def Vancomycin 0.008 mg/ml -0.6 -1.6 N.D. N.D. -5.0
nitrogen source L-Alanine (N) -0.2 -0.7 N.D. N.D. -6.2
nitrogen source Parabanic Acid (N) -0.8 -1.4 N.D. N.D. -4.9
temperature Glucose defined 30C timecourse1 timepoint 2 -0.5 -0.2 N.D. N.D. -6.2
carbon source casaminos (C) -0.5 -0.5 N.D. N.D. -5.9
stress D-Cycloserine 0.032 mg/ml -0.5 -1.4 N.D. N.D. -4.9
stress Def Polymyxin B sulfate 0.0001 mg/ml -0.9 -0.1 N.D. N.D. -5.8
carbon source a-Ketoglutaric (C) -0.6 +0.3 N.D. N.D. -6.4
carbon source D-Alanine (C) +0.1 +0.1 N.D. N.D. -6.7
carbon source L-Glutamic (C) -0.1 +0.3 N.D. N.D. -6.6
carbon source D-Maltose (C) -0.7 +0.6 N.D. N.D. -5.8
carbon source Fumarate (C) -0.2 +0.6 N.D. N.D. -6.3
carbon source L-Glutamic (C) -0.1 +0.5 N.D. N.D. -6.2
carbon source acetate (C) -0.3 +0.6 N.D. N.D. -5.9
carbon source Tween 20 (C) -0.5 +0.9 N.D. N.D. -5.9
stress nitrate 200 mM +0.6 +0.6 N.D. N.D. -5.8
carbon source L-tyrosine (C) +1.2 +1.3 N.D. N.D. -4.9
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