Fitness for 5 genes in Dechlorosoma suillum PS

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500 ntDsui_2586 and Dsui_2587 are separated by 1 nucleotidesDsui_2587 and Dsui_2588 are separated by 22 nucleotidesDsui_2588 and Dsui_2589 are separated by 10 nucleotidesDsui_2589 and Dsui_2590 are separated by 2 nucleotides Dsui_2586: Dsui_2586 - type IV secretory pathway, VirB9 component, at 2,739,072 to 2,740,058 _2586 Dsui_2587: Dsui_2587 - type IV secretory pathway, VirB10 component, at 2,740,060 to 2,741,385 _2587 Dsui_2588: Dsui_2588 - type IV secretory pathway, VirB9 component, at 2,741,408 to 2,742,262 _2588 Dsui_2589: Dsui_2589 - type II secretory pathway, component PulD, at 2,742,273 to 2,743,994 _2589 Dsui_2590: Dsui_2590 - Pilin accessory protein (PilO), at 2,743,997 to 2,745,409 _2590
Group Condition Dsui_2586 Dsui_2587 Dsui_2588 Dsui_2589 Dsui_2590
with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate -1.8 -0.7 N.D. -1.2 -2.4
No stressor control+ 10 mM ClO4 and 20 mM lactate -1.2 -0.8 N.D. -1.1 -2.6
No stressor control+ 10 mM ClO4 and 20 mM lactate -0.9 -0.8 N.D. -1.4 -2.6
with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate -1.4 -0.6 N.D. -1.3 -2.2
spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate -1.3 -0.6 N.D. -1.3 -2.3
incubated with with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate for 3 hours -0.9 -0.5 N.D. -1.6 -2.2
No stressor control+ 10 mM ClO4 and 20 mM lactate -1.2 -0.8 N.D. -1.4 -1.9
incubated with with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate for 3 hours -1.2 -0.8 N.D. -1.5 -1.7
T0 transferred and grown anaerobically in PS minimal medium+ 10 mM ClO4 and 20 mM lactate -1.1 -0.5 N.D. -1.4 -2.0
spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate -0.8 -0.5 N.D. -0.9 -2.6
alp rich rich media -1.6 -0.6 N.D. -1.0 -1.2
with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate -0.7 -0.4 N.D. -1.1 -1.5
spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate -0.3 -0.5 N.D. -1.3 -1.6
T0 transferred and grown anaerobically in PS minimal medium+ 10 mM ClO4 and 20 mM lactate -0.3 -0.0 N.D. -1.1 -2.1
carbon source defined media with 40 mM sodium acetate -1.0 -0.2 N.D. -0.8 -1.0
carbon source defined media with 30 mM sodium lactate -0.7 -0.5 N.D. -0.7 -0.8
carbon source defined media with 30 mM sodium lactate -1.2 -0.2 N.D. -0.6 -0.6
stress rich media with sodium chlorite 0.16 mM -1.1 -0.4 N.D. -0.7 -0.4
alp rich rich media -0.5 -0.6 N.D. -0.6 -0.8
carbon source defined media with 40 mM sodium acetate -0.8 -0.2 N.D. -0.5 -0.7
carbon source defined media with 40 mM sodium acetate -1.2 -0.2 N.D. -0.5 -0.3
anaerobic Anaerobic growth in rich media with perchlorate electron acceptor, 1000 uM Stressor (H2S) spiked in when cells reach OD 0.5 -1.1 +0.4 N.D. -0.1 -1.1
stress ALP with Chloride 75 mM +0.0 -1.3 N.D. -0.2 +0.7
stress ALP with Bacitracin 0.2 mg/ml +0.6 -1.2 N.D. -0.1 +0.2
carbon source D-Lactate (C) +0.2 -0.9 N.D. +0.2 +0.9
carbon source L-Lactate (C) +0.3 -0.9 N.D. +0.2 +1.1
carbon source pyruvate (C) +0.7 -0.9 N.D. +0.1 +0.8
carbon source a-Ketoglutaric (C) +0.5 -0.6 N.D. +0.4 +0.9
stress ALP with Nickel (II) chloride 2.5 mM +0.9 +0.4 N.D. +0.8 +1.5
stress ALP with copper (II) chloride 0.6 mM +1.2 +0.7 N.D. +1.2 +0.9
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