Fitness for 5 genes in Dechlorosoma suillum PS

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500 ntDsui_1515 and Dsui_1516 are separated by 70 nucleotidesDsui_1516 and Dsui_1517 are separated by 19 nucleotidesDsui_1517 and Dsui_1518 are separated by 4 nucleotidesDsui_1518 and Dsui_1519 are separated by 33 nucleotides Dsui_1515: Dsui_1515 - poly(A) polymerase, at 1,578,033 to 1,579,418 _1515 Dsui_1516: Dsui_1516 - HAD-superfamily subfamily IB hydrolase, TIGR01490, at 1,579,489 to 1,580,151 _1516 Dsui_1517: Dsui_1517 - DnaA regulatory inactivator Hda, at 1,580,171 to 1,580,854 _1517 Dsui_1518: Dsui_1518 - putative permease, at 1,580,859 to 1,581,980 _1518 Dsui_1519: Dsui_1519 - phosphoribosylaminoimidazole synthetase, at 1,582,014 to 1,583,063 _1519
Group Condition Dsui_1515 Dsui_1516 Dsui_1517 Dsui_1518 Dsui_1519
spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate -2.7 N.D. N.D. +0.1 N.D.
with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate -2.2 N.D. N.D. +0.1 N.D.
incubated with with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate for 3 hours -2.4 N.D. N.D. +0.3 N.D.
with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate -2.2 N.D. N.D. +0.2 N.D.
incubated with with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate for 3 hours -2.3 N.D. N.D. +0.3 N.D.
No stressor control+ 10 mM ClO4 and 20 mM lactate -2.4 N.D. N.D. +0.4 N.D.
spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate -2.2 N.D. N.D. +0.3 N.D.
No stressor control+ 10 mM ClO4 and 20 mM lactate -2.1 N.D. N.D. +0.3 N.D.
with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate -2.2 N.D. N.D. +0.4 N.D.
T0 transferred and grown anaerobically in PS minimal medium+ 10 mM ClO4 and 20 mM lactate -2.0 N.D. N.D. +0.4 N.D.
No stressor control+ 10 mM ClO4 and 20 mM lactate -1.8 N.D. N.D. +0.3 N.D.
spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate -1.9 N.D. N.D. +0.4 N.D.
stress ALP with copper (II) chloride 0.6 mM -1.4 N.D. N.D. -0.1 N.D.
T0 transferred and grown anaerobically in PS minimal medium+ 10 mM ClO4 and 20 mM lactate -1.9 N.D. N.D. +0.6 N.D.
anaerobic anaerobic no nitrogen ALP with 3 all (C)s and 5mM perchlorate -1.1 N.D. N.D. -0.0 N.D.
stress ALP with sodium fluoride 30 mM -1.3 N.D. N.D. +0.1 N.D.
carbon source propionate (C) -1.0 N.D. N.D. -0.2 N.D.
carbon source propionate (C) -1.0 N.D. N.D. -0.1 N.D.
carbon source defined media with 30 mM sodium lactate -1.0 N.D. N.D. +0.0 N.D.
carbon source R2A with sodium pyruvate -0.2 N.D. N.D. -0.7 N.D.
carbon source defined media with 30 mM sodium lactate -0.3 N.D. N.D. -0.5 N.D.
stress ALP with sodium fluoride 40 mM -0.8 N.D. N.D. +0.0 N.D.
stress ALP with nitrate 50 mM -0.8 N.D. N.D. +0.2 N.D.
stress rich media with sodium chlorite 0.16 mM -0.7 N.D. N.D. +0.2 N.D.
anaerobic Anaerobic, acetate donor; nitrate electron acceptor +0.6 N.D. N.D. -0.3 N.D.
anaerobic Anaerobic, acetate donor; nitrate electron acceptor +0.7 N.D. N.D. -0.2 N.D.
stress ALP with Fusidic 0.007 mg/ml +1.0 N.D. N.D. -0.1 N.D.
stress ALP with Fusidic 0.01 mg/ml +1.4 N.D. N.D. -0.2 N.D.
stress ALP with Fusidic 0.02 mg/ml +1.3 N.D. N.D. -0.1 N.D.
stress ALP with Cobalt chloride 0.25 mM +1.1 N.D. N.D. +0.3 N.D.
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