Fitness for 5 genes in Dechlorosoma suillum PS

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500 ntDsui_0001 and Dsui_0002 are separated by 67 nucleotidesDsui_0002 and Dsui_0003 are separated by 80 nucleotidesDsui_0003 and Dsui_0004 are separated by 42 nucleotidesDsui_0004 and Dsui_0005 are separated by 32 nucleotides Dsui_0001: Dsui_0001 - aminodeoxychorismate synthase, component I, at 103 to 1,923 _0001 Dsui_0002: Dsui_0002 - putative redox protein, regulator of disulfide bond formation, at 1,991 to 2,218 _0002 Dsui_0003: Dsui_0003 - putative Zn-dependent protease, at 2,299 to 3,786 _0003 Dsui_0004: Dsui_0004 - molybdenum cofactor biosynthesis protein MoaC, at 3,829 to 4,323 _0004 Dsui_0005: Dsui_0005 - putative secreted protein, at 4,356 to 4,826 _0005
Group Condition Dsui_0001 Dsui_0002 Dsui_0003 Dsui_0004 Dsui_0005
incubated with with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate for 3 hours +0.2 N.D. -2.7 -10.6 +0.7
with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate +0.2 N.D. -4.3 -8.5 +0.7
spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate +0.2 N.D. -4.4 -7.8 +0.3
No stressor control+ 10 mM ClO4 and 20 mM lactate +0.3 N.D. -2.6 -9.3 +0.6
spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate +0.4 N.D. -4.0 -7.9 +0.6
No stressor control+ 10 mM ClO4 and 20 mM lactate +0.2 N.D. -2.5 -8.9 +0.5
incubated with with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate for 3 hours +0.1 N.D. -2.8 -8.5 +0.6
with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate +0.4 N.D. -3.1 -7.5 +0.5
spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate +0.4 N.D. -3.4 -7.0 +0.6
No stressor control+ 10 mM ClO4 and 20 mM lactate +0.4 N.D. -2.9 -7.2 +0.7
with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate +0.0 N.D. -1.7 -7.2 +0.6
anaerobic Anaerobic growth in rich media with nitrate electron acceptor -0.4 N.D. -1.3 -4.7 -0.6
anaerobic Anaerobic growth in rich media with nitrate electron acceptor -0.6 N.D. -1.5 -4.0 -0.8
anaerobic Anaerobic growth in rich media with nitrate electron acceptor -0.4 N.D. -1.8 -2.8 -1.1
anaerobic Anaerobic growth in rich media with nitrate electron acceptor -0.8 N.D. -1.2 -3.0 -0.8
T0 transferred and grown anaerobically in PS minimal medium+ 10 mM ClO4 and 20 mM lactate -0.1 N.D. -2.2 -3.7 +0.4
stress ALP with Zinc 2 mM -0.3 N.D. -4.5 -0.1 +0.0
stress ALP with copper (II) chloride 0.6 mM -0.7 N.D. -3.7 +0.3 -0.4
anaerobic anaerobic ALP with 3 all (C)s and 5mM perchlorate +0.5 N.D. -2.0 -3.5 +0.6
T0 transferred and grown anaerobically in PS minimal medium+ 10 mM ClO4 and 20 mM lactate +0.4 N.D. -1.8 -3.6 +0.6
anaerobic anaerobic no nitrogen ALP with 3 all (C)s and 5mM perchlorate +0.1 N.D. -1.2 -3.7 +0.5
stress ALP with Bacitracin 0.2 mg/ml -0.2 N.D. -4.6 +0.5 +0.2
anaerobic Anaero. lactate (C), chlorate electron acceptor -0.1 N.D. -0.4 -3.8 +0.2
anaerobic Anaero. lactate (C), chlorate electron acceptor +0.2 N.D. -0.6 -3.8 +0.3
stress ALP with Nickel (II) chloride 3.5 mM -0.0 N.D. -4.0 +0.0 +0.2
stress ALP with Fusidic 0.02 mg/ml +0.1 N.D. -4.1 +0.2 +0.1
anaerobic Anaero. lactate (C), chlorate electron acceptor -0.1 N.D. -0.1 -3.9 +0.3
stress ALP with Fusidic 0.007 mg/ml +0.2 N.D. -4.1 +0.5 +0.1
stress ALP with Cobalt chloride 0.25 mM +0.1 N.D. -3.9 +0.4 +0.4
stress ALP with Nickel (II) chloride 2.5 mM +0.2 N.D. -4.1 +0.4 +0.5
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