Fitness for 5 genes in Klebsiella michiganensis M5al

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500 ntBWI76_RS27200 and BWI76_RS27205 are separated by 105 nucleotidesBWI76_RS27205 and BWI76_RS27210 are separated by 80 nucleotidesBWI76_RS27210 and BWI76_RS27215 overlap by 1 nucleotidesBWI76_RS27215 and BWI76_RS27220 are separated by 59 nucleotides BWI76_RS27200: BWI76_RS27200 - tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL, at 5,646,698 to 5,647,171 _RS27200 BWI76_RS27205: BWI76_RS27205 - serine O-acetyltransferase, at 5,647,277 to 5,648,098 _RS27205 BWI76_RS27210: BWI76_RS27210 - glycerol-3-phosphate dehydrogenase, at 5,648,179 to 5,649,195 _RS27210 BWI76_RS27215: BWI76_RS27215 - protein-export protein SecB, at 5,649,195 to 5,649,662 _RS27215 BWI76_RS27220: BWI76_RS27220 - glutaredoxin 3, at 5,649,722 to 5,649,973 _RS27220
Group Condition BWI76_RS27200 BWI76_RS27205 BWI76_RS27210 BWI76_RS27215 BWI76_RS27220
stress Cobalt chloride 0.5 mM -1.4 N.D. N.D. N.D. N.D.
mixed community LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL -1.1 N.D. N.D. N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -1.1 N.D. N.D. N.D. N.D.
ecofab LB in EcoFAB_10mL EcoFab with sand -1.0 N.D. N.D. N.D. N.D.
phage K. M5a1 KLEB22 MOI 10 -1.0 N.D. N.D. N.D. N.D.
phage KLEB17 MOI 1 -0.9 N.D. N.D. N.D. N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X -0.9 N.D. N.D. N.D. N.D.
stress RCH2_defined_1percent_glucose with Chloride 400 mM and Betaine 100 uM -0.8 N.D. N.D. N.D. N.D.
ecofab LB in EcoFAB_3.5mL -0.7 N.D. N.D. N.D. N.D.
stress RCH2_defined_1percent_glucose with Chloride 200 mM -0.7 N.D. N.D. N.D. N.D.
mixed community LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL -0.7 N.D. N.D. N.D. N.D.
exudates RCH2_defined_noCarbon with Brachypodium distachyon 27-day exudates 3 x -0.7 N.D. N.D. N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.7 N.D. N.D. N.D. N.D.
mixed community RCH2_defined_noCarbon with Mixed culture; Escherichia coli BW25113 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x -0.7 N.D. N.D. N.D. N.D.
lb dilute Dilute LB (1/10) -0.7 N.D. N.D. N.D. N.D.
micoeukaryotes Tetrahymena sp., Mixed culture-1 +0.7 N.D. N.D. N.D. N.D.
nitrogen fixation D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 3 days +0.7 N.D. N.D. N.D. N.D.
nitrogen source D-Alanine (N) +0.7 N.D. N.D. N.D. N.D.
stress Paraquat dichloride 0.1 mg/ml +0.7 N.D. N.D. N.D. N.D.
carbon source 2-Deoxyadenosine 5-monophosphate 5 mM (C) +0.7 N.D. N.D. N.D. N.D.
mixed community RCH2_defined_noCarbon with Mixed culture; Cupriavidus sp. AnTP704 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x +0.7 N.D. N.D. N.D. N.D.
stress Cobalt chloride 0.5 mM +0.7 N.D. N.D. N.D. N.D.
nitrogen source L-Cysteine (N) +0.7 N.D. N.D. N.D. N.D.
nitrogen source Casamino Acids (N) +0.7 N.D. N.D. N.D. N.D.
carbon source D-Sorbitol (C) +0.8 N.D. N.D. N.D. N.D.
nitrogen source D-Serine (N) +0.8 N.D. N.D. N.D. N.D.
exudates RCH2_defined_noCarbon with Brachypodium distachyon 27-day exudates 3 x +0.9 N.D. N.D. N.D. N.D.
carbon source D-Lactate (C) +0.9 N.D. N.D. N.D. N.D.
stress Cephalothin 0.16 mg/ml +0.9 N.D. N.D. N.D. N.D.
supernatant RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.67x +1.4 N.D. N.D. N.D. N.D.
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