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Fitness for 5 genes in
Klebsiella michiganensis M5al
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500 nt
BWI76_RS25530 and BWI76_RS25535 overlap by 28 nucleotides
BWI76_RS25535 and BWI76_RS25540 are separated by 10 nucleotides
BWI76_RS25540 and BWI76_RS25545 are separated by 110 nucleotides
BWI76_RS25545 and BWI76_RS25550 overlap by 11 nucleotides
BWI76_RS25530: BWI76_RS25530 - acetyl-CoA carboxylase, at 5,306,997 to 5,307,086
_RS25530
BWI76_RS25535: BWI76_RS25535 - acetyl-CoA carboxylase biotin carboxyl carrier protein subunit, at 5,307,059 to 5,307,532
_RS25535
BWI76_RS25540: BWI76_RS25540 - acetyl-CoA carboxylase biotin carboxylase subunit, at 5,307,543 to 5,308,892
_RS25540
BWI76_RS25545: BWI76_RS25545 - hypothetical protein, at 5,309,003 to 5,309,245
_RS25545
BWI76_RS25550: BWI76_RS25550 - sodium:pantothenate symporter, at 5,309,235 to 5,310,686
_RS25550
Group
Condition
BWI76
_RS25530
BWI76
_RS25535
BWI76
_RS25540
BWI76
_RS25545
BWI76
_RS25550
nitrogen source
L-Cysteine (N)
N.D.
N.D.
N.D.
-2.0
-0.3
nitrogen source
D-Serine (N)
N.D.
N.D.
N.D.
-1.4
-0.4
nitrogen source
L-Arginine (N)
N.D.
N.D.
N.D.
-1.8
+0.1
nitrogen fixation
D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 3 days
N.D.
N.D.
N.D.
-1.4
+0.1
phage
KLEB17 MOI 1
N.D.
N.D.
N.D.
-1.6
+0.4
stress
Cobalt chloride 0.5 mM
N.D.
N.D.
N.D.
-1.5
+0.5
carbon source
2'-Deoxycytidine 10 mM (C)
N.D.
N.D.
N.D.
-1.1
+0.2
micoeukaryotes
Dictyostellum sp.mixed culture-1
N.D.
N.D.
N.D.
-1.1
+0.2
nitrogen source
Uridine (N)
N.D.
N.D.
N.D.
-1.1
+0.5
agar plate interaction control
Parafilmed volatile agar plate with no fungus
N.D.
N.D.
N.D.
-1.1
+0.5
supernatant
RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
N.D.
N.D.
N.D.
-0.7
+0.6
mixed community
RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
N.D.
N.D.
N.D.
+1.1
-0.3
supernatant
RCH2_defined_glucose with Supernatant; Trichoderma harzianum M10 and Trichoderma harzanium TH1 and Trichoderma virens G41 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1 x and 2,2'-Dipyridyl 50 uM
N.D.
N.D.
N.D.
+1.1
-0.2
mixed community
RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
N.D.
N.D.
N.D.
+1.1
-0.2
stress
Fungal_growth_media_mCAFEs_v1 with Aluminum chloride 2 mM
N.D.
N.D.
N.D.
+1.1
-0.2
stress
Fungal_growth_media_mCAFEs_v1 with nitrate 20 mM
N.D.
N.D.
N.D.
+1.2
-0.3
supernatant
RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x
N.D.
N.D.
N.D.
+0.5
+0.8
supernatant
Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x
N.D.
N.D.
N.D.
+1.6
-0.3
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
N.D.
N.D.
N.D.
+0.8
+0.5
carbon source
2-Deoxyadenosine 5-monophosphate 5 mM (C)
N.D.
N.D.
N.D.
+1.2
+0.1
carbon source
Inosine (C)
N.D.
N.D.
N.D.
+1.0
+0.3
mixed community
LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
N.D.
N.D.
N.D.
+1.0
+0.3
mixed community
RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
N.D.
N.D.
N.D.
+1.3
+0.1
phage
KLEB17 MOI 1
N.D.
N.D.
N.D.
+1.1
+0.2
control
NoPredeControl
N.D.
N.D.
N.D.
+1.6
-0.1
mixed community
LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL
N.D.
N.D.
N.D.
+1.4
+0.1
stress
Sisomicin 0.008 mg/ml
N.D.
N.D.
N.D.
+0.7
+0.9
mixed community
RCH2_defined_noCarbon with Mixed culture; Arthrobacter sp. AnTP99 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
N.D.
N.D.
N.D.
+1.4
+0.2
stress
Sisomicin 0.008 mg/ml
N.D.
N.D.
N.D.
+1.4
+0.5
carbon source
Gly-Glu (C)
N.D.
N.D.
N.D.
+1.8
+0.3
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