Fitness for 5 genes in Klebsiella michiganensis M5al

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500 ntBWI76_RS23800 and BWI76_RS23805 are separated by 155 nucleotidesBWI76_RS23805 and BWI76_RS23810 are separated by 5 nucleotidesBWI76_RS23810 and BWI76_RS23815 are separated by 22 nucleotidesBWI76_RS23815 and BWI76_RS23820 are separated by 121 nucleotides BWI76_RS23800: BWI76_RS23800 - peptide chain release factor 2, at 4,968,113 to 4,969,141 _RS23800 BWI76_RS23805: BWI76_RS23805 - ssDNA exonuclease RecJ, at 4,969,297 to 4,971,030 _RS23805 BWI76_RS23810: BWI76_RS23810 - bifunctional protein-disulfide isomerase/oxidoreductase DsbC, at 4,971,036 to 4,971,749 _RS23810 BWI76_RS23815: BWI76_RS23815 - site-specific tyrosine recombinase XerD, at 4,971,772 to 4,972,668 _RS23815 BWI76_RS23820: BWI76_RS23820 - flavodoxin FldB, at 4,972,790 to 4,973,311 _RS23820
Group Condition BWI76_RS23800 BWI76_RS23805 BWI76_RS23810 BWI76_RS23815 BWI76_RS23820
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -0.6 -1.1 N.D. -2.0
mixed community LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL N.D. -1.0 -0.7 N.D. -1.7
phage KLEB14 MOI 0.1 N.D. -0.8 -1.6 N.D. -0.9
supernatant Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml N.D. -1.6 -0.3 N.D. -1.3
supernatant Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x N.D. -0.7 -1.3 N.D. -1.2
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB N.D. -1.2 -1.2 N.D. -0.8
phage KLEB16 MOI 0.1 N.D. -0.9 -0.5 N.D. -1.6
nitrogen source L-tyrosine (N) N.D. -0.2 -1.5 N.D. -1.0
mixed community Fungal_growth_media_mCAFEs_v1 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL N.D. -0.4 -0.9 N.D. -1.4
supernatant control Vogels_fungal_media; pH 5.5 N.D. -1.1 -0.7 N.D. -0.8
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. -0.4 -1.3 N.D. -1.0
supernatant Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml N.D. -0.5 -0.7 N.D. -1.5
supernatant control Vogels_fungal_media 0.1X N.D. -1.5 -0.3 N.D. -0.8
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. -0.9 -0.8 N.D. -0.8
stress Fungal_growth_media_mCAFEs_v1 with Vancomycin 0.01 mg/mL N.D. -0.8 -0.6 N.D. -1.0
ecofab RCH2_defined_Glucose_noNitrogen in EcoFAB_3.5mL N.D. -0.4 +0.2 N.D. -2.2
supernatant RCH2_defined_glucose with Supernatant; Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1x N.D. -0.2 -1.0 N.D. -1.1
r2a control R2A control N.D. -0.4 -0.6 N.D. -1.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant N.D. -0.4 -1.0 N.D. -0.8
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. -0.2 -1.1 N.D. -0.9
ecofab LB in EcoFAB_3.5mL with sand N.D. -0.5 -0.5 N.D. -1.1
supernatant control Vogels_fungal_media 0.5X; growth supplemented with 0.4X LB N.D. -1.1 -0.1 N.D. -0.8
supernatant RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x N.D. +0.3 -1.4 N.D. -0.9
phage K. M5a1 KLEB26 MOI 0.1 N.D. +0.4 -0.2 N.D. -2.1
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -1.8 -0.2 N.D. +0.1
mixed community RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x N.D. -0.8 +0.8 N.D. -1.4
supernatant Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM N.D. -0.7 +0.6 N.D. -1.0
rhizosphere rhizosphere EcoFAB sample 3; outgrowth in LB N.D. -1.3 +0.2 N.D. +0.8
stress Sisomicin 0.008 mg/ml N.D. -0.9 +0.6 N.D. +3.7
stress Sisomicin 0.008 mg/ml N.D. -0.5 +0.6 N.D. +4.9
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