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Fitness for 5 genes in
Klebsiella michiganensis M5al
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Top 30 experiments (either direction), sorted by average fitness
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500 nt
BWI76_RS23710 and BWI76_RS23715 are separated by 135 nucleotides
BWI76_RS23715 and BWI76_RS23720 are separated by 13 nucleotides
BWI76_RS23720 and BWI76_RS23725 are separated by 109 nucleotides
BWI76_RS23725 and BWI76_RS23730 are separated by 13 nucleotides
BWI76_RS23710: BWI76_RS23710 - 3-keto-2-deoxy-D-ribonyl-CoA thiolase
(from data)
, at 4,949,048 to 4,950,238
_RS23710
BWI76_RS23715: BWI76_RS23715 - 2-deoxy-D-ribonate transporter 1
(from data)
, at 4,950,374 to 4,951,711
_RS23715
BWI76_RS23720: BWI76_RS23720 - calcium-binding protein, at 4,951,725 to 4,952,603
_RS23720
BWI76_RS23725: BWI76_RS23725 - 2-deoxy-D-ribonate transporter 2
(from data)
, at 4,952,713 to 4,954,050
_RS23725
BWI76_RS23730: BWI76_RS23730 - glycerate kinase, at 4,954,064 to 4,955,239
_RS23730
Group
Condition
BWI76
_RS23710
BWI76
_RS23715
BWI76
_RS23720
BWI76
_RS23725
BWI76
_RS23730
carbon source
2-Deoxy-D-ribonic lithium salt 10 mM (C)
-2.9
-5.4
-1.1
-1.6
-4.9
carbon source
2-Deoxy-D-ribonic lithium salt 10 mM (C)
-3.8
-2.6
-1.3
-1.2
-4.3
micoeukaryotes
Tetrahymena sp., Mixed culture-1
-0.6
-0.7
-0.2
+0.1
-0.2
nitrogen source
Gly-DL-Asp (N)
-0.8
+0.4
-0.6
+0.0
-0.5
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant
-0.0
+0.4
-1.1
-0.0
-0.5
stress
Cobalt chloride 0.5 mM
+0.4
-0.6
-0.6
-0.3
+0.3
mixed community
RCH2_defined_noCarbon with Mixed culture; Pseudomonas simiae WCS417 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
+0.5
-0.5
-0.5
+0.2
-0.1
mixed community
RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
+0.6
-0.8
+0.0
+0.1
-0.3
mixed community
LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL
+0.7
-0.2
-0.3
-0.1
-0.4
stress
Sisomicin 0.008 mg/ml
-0.3
-0.2
-0.3
+0.3
+0.5
phage
K. M5a1 KLEB23 MOI 10
+0.8
-0.2
-0.3
-0.1
-0.1
nitrogen source
Glucuronamide (N)
+0.5
+0.2
-0.6
-0.2
+0.3
mixed community
RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
+0.4
-0.4
-0.1
+0.7
-0.2
mixed community
Fungal_growth_media_mCAFEs_v1 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
+0.9
-0.4
-0.3
+0.3
-0.0
mixed community
Fungal_growth_media_mCAFEs_v1 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL
+0.9
-0.1
-0.1
+0.2
-0.4
exudates
RCH2_defined_noCarbon with Brachypodium distachyon 27-day exudates 3 x
+0.7
-0.3
+0.6
-0.3
+0.0
micoeukaryotes
Acanthamoeba sp., muxed culture-2
-0.1
+0.6
+0.5
+0.1
-0.4
agar plate interaction control
Parafilmed volatile agar plate with no fungus
-0.0
+0.2
+1.2
+0.2
-0.4
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
+0.8
-0.1
+0.6
+0.2
-0.3
agar plate interaction control
Parafilmed volatile agar plate with no fungus
+0.2
+0.1
+1.5
+0.1
-0.4
carbon source
Gly-Glu (C)
+0.4
+0.3
+0.6
+0.6
-0.3
agar plate interaction control
Parafilmed volatile agar plate with no fungus
+0.2
+1.0
-0.0
-0.1
+0.4
nitrogen fixation
D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 3 days
+0.4
+0.6
+0.3
+0.0
+0.2
supernatant
RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.67x
+0.6
+0.5
+0.5
+0.1
-0.1
mixed community
RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
+0.3
+0.5
-0.0
+0.6
+0.3
supernatant
Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml
+0.6
+0.5
+0.2
+0.4
-0.0
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
+0.4
+0.3
-0.3
+0.4
+0.9
supernatant
Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x
+0.5
+0.5
+0.2
+0.5
+0.3
stress
Paraquat dichloride 0.1 mg/ml
+0.4
+0.3
+1.2
-0.1
+0.2
rhizosphere
rhizosphere sample 1; outgrowth in LB
+0.8
+0.6
+0.8
+0.2
-0.2
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