Fitness for 5 genes in Klebsiella michiganensis M5al

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500 ntBWI76_RS23250 and BWI76_RS23255 overlap by 4 nucleotidesBWI76_RS23255 and BWI76_RS23260 overlap by 10 nucleotidesBWI76_RS23260 and BWI76_RS23265 are separated by 218 nucleotidesBWI76_RS23265 and BWI76_RS23270 are separated by 227 nucleotides BWI76_RS23250: BWI76_RS23250 - hypothetical protein, at 4,850,536 to 4,850,943 _RS23250 BWI76_RS23255: BWI76_RS23255 - prepilin-type cleavage/methylation domain-containing protein, at 4,850,940 to 4,851,503 _RS23255 BWI76_RS23260: BWI76_RS23260 - prepilin-type cleavage/methylation domain-containing protein, at 4,851,494 to 4,851,961 _RS23260 BWI76_RS23265: BWI76_RS23265 - hypothetical protein, at 4,852,180 to 4,853,856 _RS23265 BWI76_RS23270: BWI76_RS23270 - phosphoenolpyruvate--protein phosphotransferase PtsP, at 4,854,084 to 4,856,330 _RS23270
Group Condition BWI76_RS23250 BWI76_RS23255 BWI76_RS23260 BWI76_RS23265 BWI76_RS23270
supernatant Supernatant; Trichoderma atroviride IMI tmk3mutant grown in Vogels_fungal_media, filtered; 0.8X -0.5 -0.6 -0.3 -8.1 +0.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.8 -0.1 -0.7 -7.4 +0.0
r2a control R2A control -0.4 -0.1 -0.0 -8.2 -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.6 -0.4 -0.6 -7.4 +0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.3 -0.7 -0.8 -7.4 +0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -1.1 -0.0 +0.1 -7.5 +0.1
r2a control R2A control -0.1 +0.3 -0.2 -8.2 -0.0
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X +0.5 -0.1 -0.2 -7.9 -0.4
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.2X -0.0 +0.4 -0.2 -8.0 +0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.2 +0.5 +0.3 -7.9 -0.4
nitrogen fixation D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 3 days +0.2 -0.3 -1.1 -4.2 -2.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB +0.3 +0.6 -0.3 -7.8 -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.2 -0.0 -0.5 -6.4 -0.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.7 +0.2 +0.2 -7.9 -0.3
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X +0.4 -0.5 +0.5 -7.0 -0.3
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 1X +0.2 -0.2 -0.2 -6.4 -0.3
supernatant Supernatant; Trichoderma atroviride IMI tmk3mutant grown in Vogels_fungal_media, filtered; 0.8X -0.4 -0.1 -0.4 -6.1 +0.2
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X -0.6 +0.1 +0.1 -6.4 +0.0
supernatant Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x +0.2 +0.3 -0.6 -5.5 -0.9
mixed community RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x -2.1 +0.2 +0.1 -4.5 -0.2
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.8X; growth supplemented with 0.4X LB +0.3 +0.5 -0.1 -6.5 -0.4
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB +0.3 +0.3 +0.1 -6.6 -0.2
supernatant control Vogels_fungal_media 0.5X; growth supplemented with 0.4X LB -0.2 +0.5 -0.1 -6.2 -0.2
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X -0.3 +0.4 -0.2 -6.1 +0.1
supernatant RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x; and D-Glucose 20mM -0.0 +0.5 -0.2 -5.3 -1.0
r2a control R2A control -0.0 +0.5 +0.1 -6.5 +0.0
supernatant RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x +0.3 +0.2 -0.5 -5.0 -1.0
supernatant control Vogels_fungal_media 0.1X +0.8 +0.2 -0.0 -6.6 -0.0
rhizosphere rhizosphere EcoFAB sample 3; outgrowth in LB +0.9 +0.7 -0.1 -6.5 +0.6
supernatant Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml +1.7 +0.5 +0.4 -4.2 +0.1
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