Fitness for 5 genes in Klebsiella michiganensis M5al

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500 ntBWI76_RS22665 and BWI76_RS22670 overlap by 4 nucleotidesBWI76_RS22670 and BWI76_RS22675 overlap by 1 nucleotidesBWI76_RS22675 and BWI76_RS22680 are separated by 19 nucleotidesBWI76_RS22680 and BWI76_RS22685 are separated by 139 nucleotides BWI76_RS22665: BWI76_RS22665 - tRNA pseudouridine(13) synthase TruD, at 4,723,857 to 4,724,906 _RS22665 BWI76_RS22670: BWI76_RS22670 - 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase, at 4,724,903 to 4,725,382 _RS22670 BWI76_RS22675: BWI76_RS22675 - 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, at 4,725,382 to 4,726,092 _RS22675 BWI76_RS22680: BWI76_RS22680 - cell division protein FtsB, at 4,726,112 to 4,726,429 _RS22680 BWI76_RS22685: BWI76_RS22685 - hypothetical protein, at 4,726,569 to 4,726,895 _RS22685
Group Condition BWI76_RS22665 BWI76_RS22670 BWI76_RS22675 BWI76_RS22680 BWI76_RS22685
stress Cobalt chloride 0.5 mM -0.5 N.D. N.D. N.D. -1.4
phage K. M5a1 KLEB10 MOI 10 -0.8 N.D. N.D. N.D. -0.9
stress Cobalt chloride 0.5 mM -1.0 N.D. N.D. N.D. -0.6
nitrogen source Thymidine (N) -0.1 N.D. N.D. N.D. -1.4
phage K. M5a1 KLEB10 MOI 1 -0.3 N.D. N.D. N.D. -1.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.4 N.D. N.D. N.D. -0.8
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.3 N.D. N.D. N.D. -0.9
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.5 N.D. N.D. N.D. -0.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.3 N.D. N.D. N.D. -0.8
agar plate interaction Taped volatile agar plate with Trichoderma atroviridae IMI -0.4 N.D. N.D. N.D. -0.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.1 N.D. N.D. N.D. -1.0
stress Fungal_growth_media_mCAFEs_v1 with nitrate 20 mM +0.1 N.D. N.D. N.D. -1.0
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.3 N.D. N.D. N.D. -0.8
mixed community RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x +0.3 N.D. N.D. N.D. -0.8
supernatant Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM -0.4 N.D. N.D. N.D. +1.0
supernatant Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.67x; and D-Glucose 37 mM +0.1 N.D. N.D. N.D. +1.0
supernatant RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x +0.3 N.D. N.D. N.D. +0.8
supernatant Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM +0.3 N.D. N.D. N.D. +0.9
supernatant RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x; and D-Glucose 20mM +0.4 N.D. N.D. N.D. +0.7
ecofab LB in EcoFAB_10mL +0.3 N.D. N.D. N.D. +0.9
supernatant RCH2_defined_glucose with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5 x; and 2,2'-Dipyridyl 50 uM +0.2 N.D. N.D. N.D. +1.0
supernatant Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.67x; and D-Glucose 37 mM +0.1 N.D. N.D. N.D. +1.1
supernatant RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x +0.0 N.D. N.D. N.D. +1.3
supernatant Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM +0.1 N.D. N.D. N.D. +1.2
supernatant RCH2_defined_glucose with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5 x; and 2,2'-Dipyridyl 50 uM +0.1 N.D. N.D. N.D. +1.4
ecofab LB in EcoFAB_3.5mL +0.5 N.D. N.D. N.D. +1.0
mixed community LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL +0.4 N.D. N.D. N.D. +1.2
stress Cephalothin 0.16 mg/ml +0.2 N.D. N.D. N.D. +1.9
stress Cephalothin 0.16 mg/ml +0.5 N.D. N.D. N.D. +1.7
carbon source Gly-Glu (C) +0.1 N.D. N.D. N.D. +4.3
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