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Fitness for 5 genes in
Klebsiella michiganensis M5al
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500 nt
BWI76_RS22375 and BWI76_RS22380 are separated by 189 nucleotides
BWI76_RS22380 and BWI76_RS22385 are separated by 54 nucleotides
BWI76_RS22385 and BWI76_RS22390 are separated by 12 nucleotides
BWI76_RS22390 and BWI76_RS22395 are separated by 223 nucleotides
BWI76_RS22375: BWI76_RS22375 - pectin acetylesterase, at 4,664,043 to 4,664,990
_RS22375
BWI76_RS22380: BWI76_RS22380 - 6-phospho-beta-glucosidase, at 4,665,180 to 4,666,580
_RS22380
BWI76_RS22385: BWI76_RS22385 - beta-1,4 mannanase, at 4,666,635 to 4,668,830
_RS22385
BWI76_RS22390: BWI76_RS22390 - maltoporin, at 4,668,843 to 4,670,186
_RS22390
BWI76_RS22395: BWI76_RS22395 - hypothetical protein, at 4,670,410 to 4,671,681
_RS22395
Group
Condition
BWI76
_RS22375
BWI76
_RS22380
BWI76
_RS22385
BWI76
_RS22390
BWI76
_RS22395
nitrogen source
L-Cysteine (N)
-0.5
-0.5
-0.3
-0.4
-0.0
carbon source
D-Alanine (C)
-0.7
-0.0
-0.3
-0.5
-0.1
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
-1.1
-0.1
+0.3
-0.0
-0.6
phage
KLEB16 MOI 10
+0.2
-0.9
+0.1
-0.1
-0.3
mixed community
LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL
-0.9
+0.1
+0.1
-0.6
+0.5
supernatant
Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.67x; and D-Glucose 37 mM
-0.2
-0.6
+0.4
-0.2
-0.2
supernatant control
Vogels_fungal_media; pH 5.5
+0.5
-0.5
-0.2
-0.5
+0.1
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
-0.5
-0.3
-0.2
+0.5
-0.1
mixed community
LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL
-0.7
+0.4
-0.2
-0.2
+0.3
nitrogen fixation
L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days
-0.5
-0.4
+0.4
-0.0
+0.4
agar plate interaction control
Parafilmed volatile agar plate with no fungus
-0.9
+0.5
+0.2
+0.5
-0.5
mixed community
RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
-0.3
+0.6
+0.1
-0.7
+0.1
nitrogen source
Thymine (N)
+0.2
-0.2
+0.6
-0.3
-0.3
supernatant
RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.67x
-0.3
+0.4
+0.1
-0.5
+0.4
mixed community
Fungal_growth_media_mCAFEs_v1 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL
-0.5
+0.5
+0.1
+0.2
+0.3
mixed community
RCH2_defined_noCarbon with Mixed culture; Dyella japonica UNC79MFTsu3.2 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
-0.2
+0.6
+0.4
-0.3
+0.2
agar plate interaction control
Parafilmed volatile agar plate with no fungus
+1.1
-0.3
-0.3
+0.0
+0.2
stress
Fungal_growth_media_mCAFEs_v1 with Thallium(I) acetate 0.05 mg/mL
+0.5
-0.5
+0.2
+0.2
+0.3
ecofab
RCH2_defined_Glucose_noNitrogen in EcoFAB_3.5mL
+0.3
-0.3
+0.4
+0.1
+0.6
rhizosphere
rhizosphere EcoFAB sample 3; outgrowth in LB
+1.0
-0.1
+0.1
+0.3
-0.4
phage
KLEB31 MOI 1
+0.6
+0.5
+0.2
-0.3
+0.3
micoeukaryotes
Tetrahymena sp., Mixed culture-1
+0.4
+0.4
+0.2
-0.3
+0.6
micoeukaryotes
Acanthamoeba sp., muxed culture-2
+0.6
-0.2
+0.4
-0.0
+0.6
mixed community
LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
+0.2
+0.8
+0.1
-0.1
+0.5
carbon source
Gly-Glu (C)
-0.1
+1.5
+0.3
-0.0
-0.1
phage
KLEB17 MOI 1
+1.0
+0.3
+0.2
+0.3
-0.2
stress
Cobalt chloride 0.5 mM
+0.4
+0.5
+0.2
+0.4
+0.1
nitrogen source
L-Serine (N)
+0.4
+0.4
+0.2
+0.3
+0.4
mixed community
RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
-0.1
+0.6
+0.6
-0.1
+0.7
phage
B3 MOi 1
+0.4
+0.4
+0.3
+0.2
+0.5
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