Fitness for 5 genes in Klebsiella michiganensis M5al

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500 ntBWI76_RS22365 and BWI76_RS22370 are separated by 258 nucleotidesBWI76_RS22370 and BWI76_RS22375 are separated by 117 nucleotidesBWI76_RS22375 and BWI76_RS22380 are separated by 189 nucleotidesBWI76_RS22380 and BWI76_RS22385 are separated by 54 nucleotides BWI76_RS22365: BWI76_RS22365 - hydrogenase expression/formation protein HypE, at 4,660,584 to 4,661,594 _RS22365 BWI76_RS22370: BWI76_RS22370 - transcriptional regulator FhlA, at 4,661,853 to 4,663,925 _RS22370 BWI76_RS22375: BWI76_RS22375 - pectin acetylesterase, at 4,664,043 to 4,664,990 _RS22375 BWI76_RS22380: BWI76_RS22380 - 6-phospho-beta-glucosidase, at 4,665,180 to 4,666,580 _RS22380 BWI76_RS22385: BWI76_RS22385 - beta-1,4 mannanase, at 4,666,635 to 4,668,830 _RS22385
Group Condition BWI76_RS22365 BWI76_RS22370 BWI76_RS22375 BWI76_RS22380 BWI76_RS22385
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.8 +0.0 -0.6 -0.2 -0.2
mixed community LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL -1.1 +0.0 -0.6 +0.2 -0.0
phage K. M5a1 KLEB26 MOI 0.1 -1.0 -0.4 -0.4 +0.3 -0.1
mixed community LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL -0.3 -0.5 -0.9 +0.1 +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.5 -0.2 -1.1 -0.1 +0.3
supernatant RCH2_defined_glucose with Supernatant; Trichoderma harzianum M10 and Trichoderma harzanium TH1 and Trichoderma virens G41 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1 x and 2,2'-Dipyridyl 50 uM -0.5 +0.1 -1.0 +0.2 -0.1
supernatant control Vogels_fungal_media; pH 5.5 -0.4 -0.6 +0.5 -0.5 -0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.7 +0.2 -0.1 -0.6 +0.3
mixed community RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x +0.3 +0.1 -1.3 +0.0 +0.1
stress Fungal_growth_media_mCAFEs_v1 with Zinc 2 mM -0.5 -0.4 -0.1 +0.6 -0.3
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.4 +0.2 -0.9 +0.5 +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +1.2 -0.1 -0.5 -0.3 -0.2
nitrogen source Gly-DL-Asp (N) +0.2 +0.2 -0.6 +0.5 -0.2
nitrogen source Glucuronamide (N) +0.8 +0.2 -0.8 -0.0 +0.1
exudates RCH2_defined_noCarbon with Brachypodium distachyon 27-day exudates 3 x -0.4 +0.6 +0.5 -0.5 +0.1
nitrogen source Cytosine (N) +0.4 -0.3 -0.4 -0.0 +0.6
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.0 -0.0 +1.1 -0.3 -0.3
stress Cobalt chloride 0.5 mM +0.3 -0.6 +0.4 +0.5 +0.2
stress Sisomicin 0.008 mg/ml -0.5 +0.1 +0.2 +0.7 +0.3
nitrogen source Glucuronamide (N) +0.7 -0.1 +0.7 -0.6 +0.1
rhizosphere rhizosphere sample 1; outgrowth in LB +0.7 +0.3 +0.4 -0.6 +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.7 -0.3 +0.3 +0.4 -0.1
stress Fungal_growth_media_mCAFEs_v1 with Carbenicillin 0.008 mg/mL +0.2 +0.2 +0.4 +0.7 -0.3
nitrogen source Thymine (N) +0.7 +0.2 +0.2 -0.2 +0.6
carbon source Gly-Glu (C) +0.2 -0.4 -0.1 +1.5 +0.3
micoeukaryotes Dictyostellum sp.mixed culture-2 +0.6 +0.3 +0.7 +0.1 +0.0
rhizosphere rhizosphere EcoFAB sample 3; outgrowth in LB +0.3 +0.3 +1.0 -0.1 +0.1
phage KLEB17 MOI 1 +0.2 +0.2 +0.3 +0.5 +0.5
phage KLEB17 MOI 1 +0.0 +0.4 +1.0 +0.3 +0.2
phage KLEB1 MOI 10 +0.7 -0.0 +0.8 +0.3 +0.2
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