Fitness for 5 genes in Klebsiella michiganensis M5al

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500 ntBWI76_RS22170 and BWI76_RS22175 are separated by 130 nucleotidesBWI76_RS22175 and BWI76_RS22180 are separated by 70 nucleotidesBWI76_RS22180 and BWI76_RS22185 are separated by 80 nucleotidesBWI76_RS22185 and BWI76_RS22190 are separated by 203 nucleotides BWI76_RS22170: BWI76_RS22170 - alanine--tRNA ligase, at 4,622,705 to 4,625,332 _RS22170 BWI76_RS22175: BWI76_RS22175 - recombination regulator RecX, at 4,625,463 to 4,625,963 _RS22175 BWI76_RS22180: BWI76_RS22180 - recombinase RecA, at 4,626,034 to 4,627,092 _RS22180 BWI76_RS22185: BWI76_RS22185 - hypothetical protein, at 4,627,173 to 4,627,670 _RS22185 BWI76_RS22190: BWI76_RS22190 - hypothetical protein, at 4,627,874 to 4,628,066 _RS22190
Group Condition BWI76_RS22170 BWI76_RS22175 BWI76_RS22180 BWI76_RS22185 BWI76_RS22190
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. N.D. N.D. -1.2 -0.2
supernatant RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x N.D. N.D. N.D. -1.2 -0.1
carbon source 2-Deoxyadenosine 5-monophosphate 5 mM (C) N.D. N.D. N.D. -1.5 +0.2
carbon source D-Fructose (C) N.D. N.D. N.D. -1.0 -0.2
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 1X N.D. N.D. N.D. -0.6 -0.5
nitrogen source L-Cysteine (N) N.D. N.D. N.D. -1.0 -0.1
stress nitrite 40 mM N.D. N.D. N.D. -1.1 +0.1
stress Fungal_growth_media_mCAFEs_v1 with nitrate 20 mM N.D. N.D. N.D. -0.9 +0.1
mixed community Fungal_growth_media_mCAFEs_v1 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL N.D. N.D. N.D. -1.3 +0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. N.D. N.D. -0.9 +0.3
phage K. M5a1 KLEB26 MOI 0.1 N.D. N.D. N.D. -0.8 +0.3
supernatant control Vogels_fungal_media; pH 5.5 N.D. N.D. N.D. -0.8 +0.4
phage K. M5a1 KLEB22 MOI 10 N.D. N.D. N.D. -0.8 +0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. N.D. N.D. -0.5 +0.5
stress Paraquat dichloride 0.1 mg/ml N.D. N.D. N.D. +0.6 -0.5
mixed community RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x N.D. N.D. N.D. -0.4 +0.8
ecofab RCH2_defined_Glucose_noNitrogen in EcoFAB_3.5mL N.D. N.D. N.D. +0.9 -0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant N.D. N.D. N.D. +0.8 -0.2
mixed community LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL N.D. N.D. N.D. +1.0 -0.4
mixed community LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL N.D. N.D. N.D. +0.6 +0.4
phage KLEB31 MOI 1 N.D. N.D. N.D. +1.0 +0.1
carbon source D-Glucose 20 mM (C) N.D. N.D. N.D. +1.1 +0.1
phage KLEB29 MOI 1 N.D. N.D. N.D. +1.1 +0.3
rhizosphere rhizosphere EcoFAB sample 3; outgrowth in LB N.D. N.D. N.D. +1.2 +0.2
nitrogen source L-Arginine (N) N.D. N.D. N.D. +1.2 +0.3
mixed community RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x N.D. N.D. N.D. +1.2 +0.3
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. N.D. N.D. +1.5 +0.4
rhizosphere rhizosphere sample 1; outgrowth in LB N.D. N.D. N.D. +1.7 +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. N.D. N.D. +1.9 +0.0
carbon source Gly-Glu (C) N.D. N.D. N.D. +4.9 +1.6
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