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Fitness for 5 genes in
Klebsiella michiganensis M5al
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500 nt
BWI76_RS21545 and BWI76_RS21550 overlap by 36 nucleotides
BWI76_RS21550 and BWI76_RS21555 are separated by 13 nucleotides
BWI76_RS21555 and BWI76_RS21560 are separated by 86 nucleotides
BWI76_RS21560 and BWI76_RS21565 are separated by 147 nucleotides
BWI76_RS21545: BWI76_RS21545 - nucleotide exchange factor GrpE, at 4,511,372 to 4,511,962
_RS21545
BWI76_RS21550: BWI76_RS21550 - inorganic polyphosphate kinase, at 4,511,927 to 4,512,073
_RS21550
BWI76_RS21555: BWI76_RS21555 - NAD(+) kinase, at 4,512,087 to 4,512,965
_RS21555
BWI76_RS21560: BWI76_RS21560 - DNA repair protein RecN, at 4,513,052 to 4,514,713
_RS21560
BWI76_RS21565: BWI76_RS21565 - outer membrane protein assembly factor BamE, at 4,514,861 to 4,515,202
_RS21565
Group
Condition
BWI76
_RS21545
BWI76
_RS21550
BWI76
_RS21555
BWI76
_RS21560
BWI76
_RS21565
nitrogen source
Glucuronamide (N)
N.D.
N.D.
N.D.
-0.0
-5.1
stress
Cobalt chloride 0.5 mM
N.D.
N.D.
N.D.
+0.1
-4.7
nitrogen source
nitrate (N)
N.D.
N.D.
N.D.
+0.6
-5.1
supernatant
Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x
N.D.
N.D.
N.D.
-0.9
-3.6
mixed community
RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
N.D.
N.D.
N.D.
-0.8
-3.6
mixed community
RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
N.D.
N.D.
N.D.
-0.9
-3.5
stress
RCH2_defined_1percent_glucose with Chloride 600 mM
N.D.
N.D.
N.D.
-0.3
-4.1
mixed community
RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
N.D.
N.D.
N.D.
-0.9
-3.5
mixed community
RCH2_defined_noCarbon with Mixed culture; Dyella japonica UNC79MFTsu3.2 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
N.D.
N.D.
N.D.
-0.7
-3.6
mixed community
Fungal_growth_media_mCAFEs_v1 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL
N.D.
N.D.
N.D.
-0.4
-3.7
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
N.D.
N.D.
N.D.
-0.4
-3.7
supernatant
Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml
N.D.
N.D.
N.D.
-0.5
-3.5
supernatant
Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x
N.D.
N.D.
N.D.
-0.3
-3.6
nitrogen source
Casamino Acids (N)
N.D.
N.D.
N.D.
-0.4
-3.3
stress
Paraquat dichloride 0.1 mg/ml
N.D.
N.D.
N.D.
-0.3
-3.3
mixed community
RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
N.D.
N.D.
N.D.
-0.3
-3.3
phage
KLEB20 MOI 0.1
N.D.
N.D.
N.D.
-0.2
-3.2
stress
Fungal_growth_media_mCAFEs_v1 with Vancomycin 0.01 mg/mL
N.D.
N.D.
N.D.
-0.8
-2.4
stress
Fungal_growth_media_mCAFEs_v1 with Phosphomycin 0.01 mg/mL
N.D.
N.D.
N.D.
-0.6
-2.5
phage
KLEB1 MOI 0.1
N.D.
N.D.
N.D.
-0.8
-2.3
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
N.D.
N.D.
N.D.
-0.3
-2.7
stress
Chlorite 2.5 mM
N.D.
N.D.
N.D.
-0.8
-1.9
phage
KLEB16 MOI 0.1
N.D.
N.D.
N.D.
-0.5
-2.3
mixed community
RCH2_defined_noCarbon with Mixed culture; Arthrobacter sp. AnTP99 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
N.D.
N.D.
N.D.
-1.1
-1.6
nitrogen source
Uridine (N)
N.D.
N.D.
N.D.
+0.4
-2.4
agar plate interaction control
Parafilmed volatile agar plate with no fungus
N.D.
N.D.
N.D.
+0.9
-2.3
nitrogen source
D-Alanine (N)
N.D.
N.D.
N.D.
-1.3
+1.6
supernatant
Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM
N.D.
N.D.
N.D.
-0.6
+2.3
phage
K. M5a1 KLEB22 MOI 10
N.D.
N.D.
N.D.
+1.2
+2.0
phage
K. M5a1 KLEB23 MOI 1
N.D.
N.D.
N.D.
+1.4
+2.4
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