Fitness for 5 genes in Klebsiella michiganensis M5al

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500 ntBWI76_RS21310 and BWI76_RS21315 are separated by 11 nucleotidesBWI76_RS21315 and BWI76_RS21320 overlap by 4 nucleotidesBWI76_RS21320 and BWI76_RS21325 overlap by 57 nucleotidesBWI76_RS21325 and BWI76_RS21330 are separated by 50 nucleotides BWI76_RS21310: BWI76_RS21310 - DNA repair protein RecO, at 4,462,449 to 4,463,186 _RS21310 BWI76_RS21315: BWI76_RS21315 - GTPase Era, at 4,463,198 to 4,464,103 _RS21315 BWI76_RS21320: BWI76_RS21320 - ribonuclease 3, at 4,464,100 to 4,464,780 _RS21320 BWI76_RS21325: BWI76_RS21325 - hypothetical protein, at 4,464,724 to 4,464,954 _RS21325 BWI76_RS21330: BWI76_RS21330 - S26 family signal peptidase, at 4,465,005 to 4,465,979 _RS21330
Group Condition BWI76_RS21310 BWI76_RS21315 BWI76_RS21320 BWI76_RS21325 BWI76_RS21330
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -5.2 N.D. N.D. -0.7 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -5.0 N.D. N.D. -0.0 N.D.
carbon source D-Maltose (C) -4.5 N.D. N.D. -0.4 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -4.2 N.D. N.D. -0.6 N.D.
mixed community LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL -4.4 N.D. N.D. -0.4 N.D.
nitrogen source L-Serine (N) -4.8 N.D. N.D. +0.0 N.D.
stress Fungal_growth_media_mCAFEs_v1 with Vancomycin 0.01 mg/mL -4.5 N.D. N.D. -0.3 N.D.
nitrogen source L-Phenylalanine (N) -4.6 N.D. N.D. -0.1 N.D.
mixed community RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x -4.5 N.D. N.D. -0.2 N.D.
nitrogen source Thymine (N) -4.7 N.D. N.D. +0.1 N.D.
phage KLEB1 MOI 10 -4.0 N.D. N.D. -0.6 N.D.
mixed community LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL -4.5 N.D. N.D. +0.0 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.8X; growth supplemented with 0.4X LB -3.5 N.D. N.D. -0.8 N.D.
mixed community Fungal_growth_media_mCAFEs_v1 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL -3.4 N.D. N.D. -0.9 N.D.
phage K. M5a1 KLEB26 MOI 0.1 -3.5 N.D. N.D. -0.7 N.D.
mixed community LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL -4.5 N.D. N.D. +0.4 N.D.
nitrogen source L-tyrosine (N) -4.8 N.D. N.D. +0.7 N.D.
carbon source 2-Deoxy-D-ribonic lithium salt 10 mM (C) -4.0 N.D. N.D. -0.1 N.D.
nitrogen source D-Alanine (N) -4.6 N.D. N.D. +0.6 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus -2.1 N.D. N.D. -1.7 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -3.6 N.D. N.D. -0.2 N.D.
mixed community RCH2_defined_noCarbon with Mixed culture; Cupriavidus sp. AnTP704 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x -2.7 N.D. N.D. -1.0 N.D.
exudates RCH2_defined_noCarbon with Brachypodium distachyon 27-day exudates 3 x -4.5 N.D. N.D. +0.8 N.D.
phage KLEB15 MOI 10 -3.7 N.D. N.D. +0.2 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB -3.4 N.D. N.D. +0.3 N.D.
exudates RCH2_defined_noCarbon with Brachypodium distachyon 27-day exudates 3 x -3.4 N.D. N.D. +0.4 N.D.
supernatant Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml -3.3 N.D. N.D. +0.5 N.D.
mixed community RCH2_defined_noCarbon with Mixed culture; Dyella japonica UNC79MFTsu3.2 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x -3.3 N.D. N.D. +0.6 N.D.
mixed community LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL -3.4 N.D. N.D. +1.1 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -1.8 N.D. N.D. +3.8 N.D.
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