Fitness for 5 genes in Klebsiella michiganensis M5al

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500 ntBWI76_RS21275 and BWI76_RS21280 overlap by 4 nucleotidesBWI76_RS21280 and BWI76_RS21285 are separated by 19 nucleotidesBWI76_RS21285 and BWI76_RS21290 are separated by 209 nucleotidesBWI76_RS21290 and BWI76_RS21295 are separated by 166 nucleotides BWI76_RS21275: BWI76_RS21275 - lytic transglycosylase F, at 4,456,991 to 4,458,607 _RS21275 BWI76_RS21280: BWI76_RS21280 - tRNA adenosine(34) deaminase TadA, at 4,458,604 to 4,459,149 _RS21280 BWI76_RS21285: BWI76_RS21285 - HAD family hydrolase, at 4,459,169 to 4,459,804 _RS21285 BWI76_RS21290: BWI76_RS21290 - DNA-binding transcriptional regulator, at 4,460,014 to 4,460,862 _RS21290 BWI76_RS21295: BWI76_RS21295 - ferredoxin, at 4,461,029 to 4,461,289 _RS21295
Group Condition BWI76_RS21275 BWI76_RS21280 BWI76_RS21285 BWI76_RS21290 BWI76_RS21295
carbon source 2-Deoxyadenosine 5-monophosphate 5 mM (C) +0.2 N.D. -5.6 -0.9 N.D.
stress Sisomicin 0.008 mg/ml +0.4 N.D. -4.6 +0.4 N.D.
stress Cobalt chloride 0.5 mM -1.9 N.D. -1.2 +0.1 N.D.
stress Cobalt chloride 0.5 mM -2.5 N.D. -1.2 +1.3 N.D.
carbon source Gly-Glu (C) -0.2 N.D. -1.1 -0.9 N.D.
supernatant RCH2_defined_glucose with Supernatant; Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1x +0.1 N.D. -2.4 +0.1 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.2 N.D. -1.1 -0.9 N.D.
carbon source 2'-Deoxyinosine 5 mM (C) -0.4 N.D. -1.7 +0.2 N.D.
nitrogen source L-Lysine (N) -0.6 N.D. -1.2 +0.4 N.D.
mixed community LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL +1.3 N.D. -0.7 -1.7 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.6 N.D. -0.5 -1.1 N.D.
mixed community LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL +0.6 N.D. +0.5 -1.7 N.D.
phage KLEB20 MOI 1 +0.5 N.D. -1.4 +0.4 N.D.
phage KLEB14 MOI 0.1 +1.1 N.D. -0.8 -0.7 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.3 N.D. +0.9 -1.0 N.D.
mixed community LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL +1.1 N.D. -0.2 -1.2 N.D.
mixed community LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL +1.2 N.D. -0.1 -0.7 N.D.
rhizosphere rhizosphere EcoFAB sample 3; outgrowth in LB +1.2 N.D. +0.6 -1.4 N.D.
mixed community RCH2_defined_noCarbon with Mixed culture; Pseudomonas simiae WCS417 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x +1.0 N.D. +0.4 -0.9 N.D.
phage KLEB24 MOI 1 +1.1 N.D. +0.4 -0.6 N.D.
stress perchlorate 100 mM +1.3 N.D. -0.7 +0.4 N.D.
phage K. M5a1 KLEB26 MOI 0.1 +1.7 N.D. +0.5 -0.3 N.D.
phage KLEB17 MOI 1 +0.5 N.D. +0.3 +1.2 N.D.
mixed community RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x -0.2 N.D. +1.1 +1.1 N.D.
phage KLEB16 MOI 10 +0.6 N.D. +1.0 +0.5 N.D.
phage KLEB29 MOI 1 +1.4 N.D. +1.0 -0.1 N.D.
phage KLEB16 MOI 0.1 +1.1 N.D. +0.7 +0.5 N.D.
phage KLEB16 MOI 1 +1.3 N.D. +0.4 +0.5 N.D.
phage KLEB16 MOI 0.1 +1.0 N.D. +0.6 +0.8 N.D.
phage KLEB17 MOI 0.1 +0.1 N.D. +1.2 +1.4 N.D.
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