Fitness for 5 genes in Klebsiella michiganensis M5al

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500 ntBWI76_RS21075 and BWI76_RS21080 are separated by 34 nucleotidesBWI76_RS21080 and BWI76_RS21085 are separated by 91 nucleotidesBWI76_RS21085 and BWI76_RS21090 are separated by 26 nucleotidesBWI76_RS21090 and BWI76_RS21095 are separated by 275 nucleotides BWI76_RS21075: BWI76_RS21075 - membrane protein, at 4,409,228 to 4,409,848 _RS21075 BWI76_RS21080: BWI76_RS21080 - histidine--tRNA ligase, at 4,409,883 to 4,411,157 _RS21080 BWI76_RS21085: BWI76_RS21085 - 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin), at 4,411,249 to 4,412,370 _RS21085 BWI76_RS21090: BWI76_RS21090 - cytoskeletal protein RodZ, at 4,412,397 to 4,413,356 _RS21090 BWI76_RS21095: BWI76_RS21095 - bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN, at 4,413,632 to 4,414,798 _RS21095
Group Condition BWI76_RS21075 BWI76_RS21080 BWI76_RS21085 BWI76_RS21090 BWI76_RS21095
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days -4.4 N.D. N.D. -0.6 -0.3
phage KLEB15 MOI 1 -3.8 N.D. N.D. -0.2 -0.1
mixed community LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL -3.0 N.D. N.D. -0.9 -0.2
supernatant RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.25x; and D-Glucose 20mM -2.9 N.D. N.D. -1.0 -0.2
nitrogen source Gly-DL-Asp (N) -2.9 N.D. N.D. -0.4 -0.5
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.8X; growth supplemented with 0.4X LB -3.3 N.D. N.D. -0.2 -0.3
nitrogen source L-Arginine (N) -3.4 N.D. N.D. +0.3 -0.6
mixed community LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL -3.1 N.D. N.D. -0.7 +0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus -3.2 N.D. N.D. -0.2 -0.2
supernatant RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 6 x -2.9 N.D. N.D. -0.6 -0.0
micoeukaryotes Tetrahymena sp., Mixed culture-1 -3.0 N.D. N.D. +0.1 -0.6
mixed community Fungal_growth_media_mCAFEs_v1 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL -2.8 N.D. N.D. -0.8 +0.4
mixed community RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x -3.0 N.D. N.D. -0.4 +0.2
mixed community Fungal_growth_media_mCAFEs_v1 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL -3.0 N.D. N.D. -0.5 +0.3
mixed community LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL -3.0 N.D. N.D. -0.8 +0.6
mixed community Fungal_growth_media_mCAFEs_v1 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL -3.0 N.D. N.D. -0.4 +0.3
mixed community Fungal_growth_media_mCAFEs_v1 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL -3.2 N.D. N.D. -0.2 +0.4
mixed community LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL -2.8 N.D. N.D. -0.6 +0.5
mixed community Fungal_growth_media_mCAFEs_v1 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL -2.9 N.D. N.D. -0.4 +0.5
agar plate interaction control Parafilmed volatile agar plate with no fungus -2.4 N.D. N.D. -0.7 +0.7
mixed community Fungal_growth_media_mCAFEs_v1 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL -3.0 N.D. N.D. +0.1 +0.5
stress Fungal_growth_media_mCAFEs_v1 with Aluminum chloride 2 mM -3.1 N.D. N.D. +0.2 +0.8
stress Fungal_growth_media_mCAFEs_v1 with Vancomycin 0.01 mg/mL -3.0 N.D. N.D. -0.1 +1.3
stress Fungal_growth_media_mCAFEs_v1 with Thallium(I) acetate 0.05 mg/mL -2.9 N.D. N.D. +0.7 +0.5
stress Fungal_growth_media_mCAFEs_v1 with Thallium(I) acetate 0.05 mg/mL -2.4 N.D. N.D. +0.5 +0.8
stress Cephalothin 0.16 mg/ml +1.8 N.D. N.D. -0.3 -1.8
stress Sisomicin 0.008 mg/ml -1.7 N.D. N.D. +0.6 +1.8
phage KLEB30 MOI 1 +2.4 N.D. N.D. +0.8 -0.5
phage KLEB20 MOI 0.1 +2.7 N.D. N.D. -0.5 +0.6
phage KLEB31 MOI 1 +3.5 N.D. N.D. +0.2 -0.3
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