Fitness for 5 genes in Klebsiella michiganensis M5al

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500 ntBWI76_RS20590 and BWI76_RS20595 are separated by 89 nucleotidesBWI76_RS20595 and BWI76_RS20600 are separated by 96 nucleotidesBWI76_RS20600 and BWI76_RS20605 are separated by 60 nucleotidesBWI76_RS20605 and BWI76_RS20610 are separated by 122 nucleotides BWI76_RS20590: BWI76_RS20590 - tRNA-Lys, at 4,309,321 to 4,309,396 _RS20590 BWI76_RS20595: BWI76_RS20595 - LysR family transcriptional regulator, at 4,309,486 to 4,310,370 _RS20595 BWI76_RS20600: BWI76_RS20600 - nucleoside permease, at 4,310,467 to 4,311,723 _RS20600 BWI76_RS20605: BWI76_RS20605 - purine-nucleoside phosphorylase, at 4,311,784 to 4,312,617 _RS20605 BWI76_RS20610: BWI76_RS20610 - LysR family transcriptional regulator, at 4,312,740 to 4,313,666 _RS20610
Group Condition BWI76_RS20590 BWI76_RS20595 BWI76_RS20600 BWI76_RS20605 BWI76_RS20610
phage KLEB1 MOI 10 N.D. -0.2 -0.2 -1.3 -0.3
phage KLEB26 MOI0.1 N.D. -0.1 -0.4 -0.6 -0.8
stress Cobalt chloride 0.5 mM N.D. -0.4 +0.0 -0.0 -1.4
phage KLEB1 MOI 10 N.D. +0.1 -0.2 -1.6 +0.0
phage KLEB29 MOI 1 N.D. -0.2 -0.1 -0.6 -0.8
phage K. M5a1 KLEB26 MOI 0.1 N.D. -0.3 -0.6 -0.9 +0.1
phage KLEB31 MOI 1 N.D. +0.3 +0.0 -0.6 -0.7
carbon source 2-Deoxyadenosine 4 mM (C) N.D. -0.1 +0.4 +0.0 -1.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. +0.9 -0.5 -1.3 +0.2
nitrogen source D-Serine (N) N.D. -0.6 -0.2 +0.6 -0.3
nitrogen source Glucuronamide (N) N.D. +0.5 -0.1 -1.3 +0.5
micoeukaryotes Acanthamoeba sp., muxed culture-2 N.D. -0.8 +0.3 -0.9 +1.1
phage B3 MOi 1 N.D. -0.2 -0.0 -0.6 +0.8
carbon source D-Glucose 20 mM (C) N.D. +0.5 +0.2 +0.4 -1.1
supernatant Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x N.D. +0.1 -0.4 +0.8 -0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant N.D. +0.6 +0.1 -0.9 +0.7
phage K. M5a1 KLEB23 MOI 10 N.D. -0.7 +0.1 +1.0 +0.3
mixed community LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL N.D. +0.3 +0.1 +0.6 -0.4
phage K. M5a1 KLEB22 MOI 10 N.D. -0.2 -0.2 +0.6 +0.5
stress Fungal_growth_media_mCAFEs_v1 with Thallium(I) acetate 0.05 mg/mL N.D. +0.5 +0.3 +0.3 -0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. +0.1 -0.2 +0.8 +0.6
phage K. M5a1 KLEB23 MOI 1 N.D. +0.0 -0.2 +0.5 +0.8
ecofab RCH2_defined_Glucose_noNitrogen in EcoFAB_3.5mL N.D. -0.2 +0.2 +0.7 +0.6
stress Chloramphenicol 0.001 mg/ml N.D. +0.2 -0.1 +0.4 +1.0
phage K. M5a1 KLEB22 MOI 10 N.D. -0.1 +0.1 +0.8 +0.5
stress Fungal_growth_media_mCAFEs_v1 with Vancomycin 0.01 mg/mL N.D. +0.4 -0.0 +0.6 +0.6
carbon source Inosine (C) N.D. +0.6 +0.2 +0.4 +0.5
supernatant Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM N.D. +0.3 +0.1 +0.4 +0.9
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. +0.6 -0.0 +0.3 +0.9
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. +0.0 +0.0 +0.1 +1.7
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