Fitness for 5 genes in Klebsiella michiganensis M5al

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500 ntBWI76_RS20260 and BWI76_RS20265 are separated by 79 nucleotidesBWI76_RS20265 and BWI76_RS20270 are separated by 236 nucleotidesBWI76_RS20270 and BWI76_RS20275 are separated by 2 nucleotidesBWI76_RS20275 and BWI76_RS20280 are separated by 58 nucleotides BWI76_RS20260: BWI76_RS20260 - PTS mannitol transporter subunit IIB, at 4,247,032 to 4,247,304 _RS20260 BWI76_RS20265: BWI76_RS20265 - sugar phosphotransferase subunit IIA, at 4,247,384 to 4,247,827 _RS20265 BWI76_RS20270: BWI76_RS20270 - hypothetical protein, at 4,248,064 to 4,249,083 _RS20270 BWI76_RS20275: BWI76_RS20275 - NUDIX hydrolase, at 4,249,086 to 4,249,640 _RS20275 BWI76_RS20280: BWI76_RS20280 - phosphodiesterase, at 4,249,699 to 4,250,250 _RS20280
Group Condition BWI76_RS20260 BWI76_RS20265 BWI76_RS20270 BWI76_RS20275 BWI76_RS20280
rhizosphere rhizosphere EcoFAB sample 3; outgrowth in LB -0.8 -0.8 -8.2 N.D. +0.0
carbon source D-Alanine (C) -0.0 +0.0 -6.5 N.D. -0.2
rhizosphere rhizosphere sample 1; outgrowth in LB -0.7 -1.1 -4.5 N.D. +0.1
carbon source D-Lactate (C) -0.1 +0.2 -5.2 N.D. -0.1
carbon source succinate (C) +0.1 +0.1 -4.8 N.D. -0.3
carbon source L-Glutamine (C) +0.1 +0.1 -4.2 N.D. +0.0
carbon source D-Alanine (C) +0.1 +0.3 -3.8 N.D. -0.2
mixed community LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL -0.1 -0.2 -1.5 N.D. -1.8
mixed community RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x -0.3 -0.6 -0.4 N.D. -2.1
mixed community RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x -0.3 -0.3 -0.8 N.D. -1.9
carbon source succinate (C) +0.1 +0.1 -3.4 N.D. +0.1
mixed community RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x +0.0 -0.2 -0.8 N.D. -2.1
supernatant Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x -0.1 -0.3 -0.8 N.D. -2.0
supernatant RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 6 x -0.3 -0.3 -1.3 N.D. -1.2
carbon source L-Alanine (C) +0.1 +0.1 -2.9 N.D. -0.4
mixed community RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x -0.1 -0.5 -0.7 N.D. -1.6
carbon source Inosine (C) -0.0 -0.1 -2.6 N.D. -0.2
mixed community RCH2_defined_noCarbon with Mixed culture; Arthrobacter sp. AnTP99 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x -0.3 -0.3 -0.8 N.D. -1.4
carbon source L-Alanine (C) -0.1 +0.2 -2.6 N.D. -0.2
mixed community LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL -0.1 -0.1 -1.0 N.D. -1.4
carbon source D-Lactate (C) -0.1 +0.0 -2.4 N.D. -0.1
mixed community RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x -0.0 -0.4 -1.0 N.D. -1.2
carbon source Gly-Glu (C) +0.1 +0.2 -2.2 N.D. -0.7
mixed community RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x -0.3 -0.4 -0.8 N.D. -1.2
agar plate interaction Taped volatile agar plate with Trichoderma atroviridae IMI +0.2 -0.3 -1.0 N.D. -1.2
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.2 -0.3 -2.1 N.D. +0.3
carbon source Inosine (C) +0.2 +0.2 -2.7 N.D. +0.1
supernatant RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x -0.1 -0.1 -2.0 N.D. +0.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.2 +0.5 -1.4 N.D. +0.5
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.7 +0.2 -1.2 N.D. +0.6
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