Fitness for 5 genes in Klebsiella michiganensis M5al

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500 ntBWI76_RS18850 and BWI76_RS18855 are separated by 30 nucleotidesBWI76_RS18855 and BWI76_RS18860 are separated by 14 nucleotidesBWI76_RS18860 and BWI76_RS18865 overlap by 1 nucleotidesBWI76_RS18865 and BWI76_RS18870 overlap by 4 nucleotides BWI76_RS18850: BWI76_RS18850 - iron-sulfur cluster assembly scaffold protein NifU, at 3,910,031 to 3,910,855 _RS18850 BWI76_RS18855: BWI76_RS18855 - cysteine desulfurase NifS, at 3,910,886 to 3,912,088 _RS18855 BWI76_RS18860: BWI76_RS18860 - homocitrate synthase, at 3,912,103 to 3,913,245 _RS18860 BWI76_RS18865: BWI76_RS18865 - nitrogenase-stabilizing/protective protein NifW, at 3,913,245 to 3,913,505 _RS18865 BWI76_RS18870: BWI76_RS18870 - protein NifZ, at 3,913,502 to 3,913,948 _RS18870
Group Condition BWI76_RS18850 BWI76_RS18855 BWI76_RS18860 BWI76_RS18865 BWI76_RS18870
nitrogen fixation D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 3 days -2.1 -2.2 -2.3 N.D. -2.2
nitrogen fixation D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 8 days -1.4 -1.3 -1.7 N.D. -1.1
nitrogen source RCH2_defined_Glucose_noNitrogen in Eppendorf tube with 0.1 mM glass beads -1.1 -0.3 -1.0 N.D. -2.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.0 -0.4 -0.8 N.D. -3.4
nitrogen source L-Arginine (N) -0.1 -0.3 -0.1 N.D. -3.9
nitrogen source RCH2_defined_Glucose_noNitrogen in Eppendorf tube with sand -1.3 -0.5 -1.1 N.D. -1.5
nitrogen source RCH2_defined_Glucose_noNitrogen in Eppendorf tube -0.7 -0.9 -1.1 N.D. -1.6
nitrogen source Glucuronamide (N) -0.0 -2.4 -0.2 N.D. -0.6
phage K. M5a1 KLEB22 MOI 10 -0.4 -0.4 +0.1 N.D. -2.5
nitrogen source Uridine (N) +0.1 -0.6 -0.0 N.D. -2.5
supernatant control Vogels_fungal_media; pH 5.5 -0.2 -0.9 -0.3 N.D. -1.6
nitrogen source D-Alanine (N) -0.1 -1.4 -0.4 N.D. -1.0
nitrogen source L-Threonine (N) -0.0 -0.9 +0.2 N.D. -1.8
nitrogen source nitrate (N) -0.2 -1.6 +0.1 N.D. -0.8
supernatant Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml +0.4 -0.5 -0.5 N.D. -1.9
carbon source D-Gluconic Acid (C) +0.0 -0.9 +0.2 N.D. -1.7
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.0 -0.6 +0.5 N.D. -1.9
stress Fungal_growth_media_mCAFEs_v1 with Aluminum chloride 2 mM +0.3 -0.4 -0.1 N.D. -1.6
nitrogen source Thymidine (N) -0.6 -0.8 -0.7 N.D. +0.4
phage K. M5a1 KLEB23 MOI 10 -0.5 +0.6 -0.2 N.D. -1.3
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.3 +0.7 -0.3 N.D. -1.3
phage KLEB1 MOI 10 +0.2 +0.6 +0.2 N.D. -1.8
nitrogen source Glucuronamide (N) -0.2 -1.0 -0.3 N.D. +0.8
phage K. M5a1 KLEB23 MOI 1 -0.1 -0.9 -0.5 N.D. +1.0
supernatant RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.67x +0.5 +1.2 -0.3 N.D. -0.6
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.1 +1.3 +0.9 N.D. -1.1
phage KLEB29 MOI 1 +0.3 -0.9 +0.7 N.D. +1.1
supernatant RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.67x +0.3 +0.0 -0.1 N.D. +2.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.1 +1.5 +0.5 N.D. +0.5
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.2 +0.8 -0.0 N.D. +2.1
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