Fitness for 5 genes in Klebsiella michiganensis M5al

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500 ntBWI76_RS18795 and BWI76_RS18800 are separated by 1 nucleotidesBWI76_RS18800 and BWI76_RS18805 are separated by 7 nucleotidesBWI76_RS18805 and BWI76_RS18810 are separated by 404 nucleotidesBWI76_RS18810 and BWI76_RS18815 are separated by 16 nucleotides BWI76_RS18795: BWI76_RS18795 - hypothetical protein, at 3,896,956 to 3,897,150 _RS18795 BWI76_RS18800: BWI76_RS18800 - transcriptional regulator, at 3,897,152 to 3,897,463 _RS18800 BWI76_RS18805: BWI76_RS18805 - pyruvate:ferredoxin (flavodoxin) oxidoreductase, at 3,897,471 to 3,900,986 _RS18805 BWI76_RS18810: BWI76_RS18810 - nitrogenase iron protein, at 3,901,391 to 3,902,272 _RS18810 BWI76_RS18815: BWI76_RS18815 - nitrogenase molybdenum-iron protein alpha chain, at 3,902,289 to 3,903,737 _RS18815
Group Condition BWI76_RS18795 BWI76_RS18800 BWI76_RS18805 BWI76_RS18810 BWI76_RS18815
nitrogen fixation D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 8 days N.D. -0.4 -1.4 -1.5 -1.8
exudates RCH2_defined_noCarbon with Brachypodium distachyon 27-day exudates 3 x N.D. +0.4 +0.6 +0.0 -5.0
nitrogen fixation D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 3 days N.D. +0.4 -1.6 -1.6 -1.2
nitrogen source RCH2_defined_Glucose_noNitrogen in Eppendorf tube N.D. -0.3 -1.2 -0.3 -1.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -0.4 +0.1 +0.6 -3.6
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -0.6 -0.3 +0.4 -2.5
carbon source RCH2_defined_noCarbon with Glu_DLac_Acetate_mix 0.1 x N.D. +0.2 -0.0 +0.6 -3.3
mixed community RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x N.D. +0.1 -0.1 +0.9 -3.2
mixed community RCH2_defined_noCarbon with Mixed culture; Pseudomonas simiae WCS417 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x N.D. +0.3 +0.1 -0.0 -2.4
mixed community LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL N.D. +0.9 -0.0 +0.6 -3.3
mixed community LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL N.D. +0.4 -0.2 +0.4 -2.3
mixed community LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL N.D. +0.9 -0.4 -0.1 -2.1
supernatant RCH2_defined_glucose with Supernatant; Trichoderma harzianum M10 and Trichoderma harzanium TH1 and Trichoderma virens G41 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1 x and Iron (II) 0.002 mg/ml N.D. +0.3 -0.0 +0.6 -2.4
stress Fungal_growth_media_mCAFEs_v1 with Zinc 2 mM N.D. +0.7 +0.2 +1.3 -3.5
mixed community LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL N.D. +1.4 -0.1 +0.0 -2.7
mixed community RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x N.D. +0.4 -0.0 +0.8 -2.3
mixed community Fungal_growth_media_mCAFEs_v1 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL N.D. +0.4 -0.1 +0.7 -2.0
mixed community LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL N.D. +1.4 +0.1 +0.2 -2.7
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. +0.6 -0.1 +0.4 -1.7
mixed community RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x N.D. +0.4 -0.1 +0.8 -1.7
stress Fungal_growth_media_mCAFEs_v1 with Zinc 2 mM N.D. +0.5 +0.2 +0.7 -1.8
mixed community Fungal_growth_media_mCAFEs_v1 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL N.D. +0.6 +0.2 +0.6 -1.7
supernatant Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x N.D. +0.2 +0.4 +1.0 -1.8
mixed community LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL N.D. +0.5 -0.2 +0.9 -1.4
mixed community LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL N.D. +0.6 +0.1 +0.8 -1.5
mixed community LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL N.D. +0.4 +0.1 +0.8 -1.3
supernatant Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x N.D. +0.5 +0.3 +0.7 -1.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. +1.5 +0.7 +0.2 -0.8
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant N.D. +2.7 -0.2 +0.1 +1.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. +3.8 -0.3 +0.8 +0.2
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