Fitness for 5 genes in Klebsiella michiganensis M5al

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500 ntBWI76_RS18740 and BWI76_RS18745 are separated by 185 nucleotidesBWI76_RS18745 and BWI76_RS18750 are separated by 131 nucleotidesBWI76_RS18750 and BWI76_RS18755 are separated by 39 nucleotidesBWI76_RS18755 and BWI76_RS18760 are separated by 193 nucleotides BWI76_RS18740: BWI76_RS18740 - HPP family protein, at 3,884,714 to 3,885,445 _RS18740 BWI76_RS18745: BWI76_RS18745 - adenosylhomocysteinase, at 3,885,631 to 3,886,740 _RS18745 BWI76_RS18750: BWI76_RS18750 - adenosylcobinamide-GDP ribazoletransferase, at 3,886,872 to 3,887,612 _RS18750 BWI76_RS18755: BWI76_RS18755 - adenosylcobinamide kinase/adenosylcobinamide-phosphate guanylyltransferase, at 3,887,652 to 3,887,885 _RS18755 BWI76_RS18760: BWI76_RS18760 - hypothetical protein, at 3,888,079 to 3,888,414 _RS18760
Group Condition BWI76_RS18740 BWI76_RS18745 BWI76_RS18750 BWI76_RS18755 BWI76_RS18760
supernatant RCH2_defined_glucose with Supernatant; Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1x -0.2 +0.0 N.D. -5.0 N.D.
supernatant RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.25x; and D-Glucose 20mM -0.3 +0.6 N.D. -5.4 N.D.
phage K. M5a1 KLEB26 MOI 0.1 -0.0 -0.7 N.D. -4.2 N.D.
nitrogen source L-tyrosine (N) +0.7 +0.4 N.D. -5.3 N.D.
carbon source 2-Deoxyadenosine 4 mM (C) -0.2 +0.6 N.D. -4.5 N.D.
phage K. M5a1 KLEB23 MOI 10 -1.1 -0.6 N.D. -1.4 N.D.
supernatant RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x -0.0 -0.2 N.D. -2.7 N.D.
phage KLEB15 MOI 10 -0.0 -1.2 N.D. -1.6 N.D.
stress Fungal_growth_media_mCAFEs_v1 with nitrate 20 mM -0.5 -0.7 N.D. -1.6 N.D.
nitrogen source L-Phenylalanine (N) -1.3 -0.2 N.D. -1.3 N.D.
nitrogen source L-Arginine (N) -0.3 -0.9 N.D. -1.4 N.D.
mixed community RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x +0.4 -0.6 N.D. -2.2 N.D.
phage KLEB1 MOI 10 +0.6 +0.1 N.D. -3.0 N.D.
phage KLEB30 MOI 1 +0.6 -0.1 N.D. -2.3 N.D.
agar plate interaction Taped volatile agar plate with Trichoderma atroviridae IMI +0.8 -0.1 N.D. -2.0 N.D.
ecofab RCH2_defined_Glucose_noNitrogen in EcoFAB_3.5mL +0.3 +0.9 N.D. -2.2 N.D.
phage KLEB1 MOI 10 +0.8 +0.5 N.D. -1.7 N.D.
stress Paraquat dichloride 0.1 mg/ml -0.1 -1.5 N.D. +1.4 N.D.
supernatant Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml -1.5 +0.7 N.D. +0.7 N.D.
mixed community Fungal_growth_media_mCAFEs_v1 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL -0.3 -0.8 N.D. +1.6 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.2 +2.1 N.D. -0.7 N.D.
stress Cobalt chloride 0.5 mM +0.1 -1.1 N.D. +2.3 N.D.
carbon source Gly-Glu (C) -0.7 +2.6 N.D. -0.1 N.D.
mixed community RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x +0.4 +0.9 N.D. +1.4 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.5 +0.2 N.D. +2.3 N.D.
phage B3 MOi 1 -0.1 +0.8 N.D. +2.2 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +1.2 +0.5 N.D. +1.4 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.8 +2.6 N.D. -0.2 N.D.
phage KLEB31 MOI 1 +0.2 +0.3 N.D. +3.1 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.4 +1.3 N.D. +3.3 N.D.
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