Fitness for 5 genes in Klebsiella michiganensis M5al

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500 ntBWI76_RS17965 and BWI76_RS17970 are separated by 74 nucleotidesBWI76_RS17970 and BWI76_RS17975 are separated by 236 nucleotidesBWI76_RS17975 and BWI76_RS17980 are separated by 19 nucleotidesBWI76_RS17980 and BWI76_RS17985 are separated by 97 nucleotides BWI76_RS17965: BWI76_RS17965 - MFS transporter, at 3,731,475 to 3,732,848 _RS17965 BWI76_RS17970: BWI76_RS17970 - zinc metalloprotease HtpX, at 3,732,923 to 3,733,804 _RS17970 BWI76_RS17975: BWI76_RS17975 - tail-specific protease, at 3,734,041 to 3,736,089 _RS17975 BWI76_RS17980: BWI76_RS17980 - RNA chaperone ProQ, at 3,736,109 to 3,736,792 _RS17980 BWI76_RS17985: BWI76_RS17985 - hypothetical protein, at 3,736,890 to 3,737,387 _RS17985
Group Condition BWI76_RS17965 BWI76_RS17970 BWI76_RS17975 BWI76_RS17980 BWI76_RS17985
carbon source Mix of RCH2_defined_noCarbon and Fungal_growth_media_mCAFEs_v1_noCarbon; D-Glucose at 55.5 mM -0.2 -0.1 -6.0 -5.3 +0.2
nitrogen source L-tyrosine (N) +0.3 -4.5 -2.7 -3.0 -0.3
mixed community Fungal_growth_media_mCAFEs_v1 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL -0.0 -0.3 -5.5 -3.7 +0.2
mixed community LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL +0.5 +0.5 -5.3 -5.1 +0.1
mixed community LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL -0.3 +0.6 -4.8 -5.0 +0.2
mixed community Fungal_growth_media_mCAFEs_v1 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL +0.3 -1.0 -5.4 -4.2 +1.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB +0.2 -0.2 -4.9 -3.9 -0.3
stress Fungal_growth_media_mCAFEs_v1 with nitrate 20 mM -0.1 -0.2 -3.8 -4.8 -0.1
stress Fungal_growth_media_mCAFEs_v1 with Aluminum chloride 2 mM +0.4 +0.4 -4.3 -5.0 -0.2
mixed community Fungal_growth_media_mCAFEs_v1 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL +0.6 -0.2 -5.3 -4.1 +0.4
mixed community RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x -0.1 +0.5 -5.2 -3.9 +0.2
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X +0.1 +0.1 -5.0 -3.6 -0.1
mixed community RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x -0.4 +0.3 -4.9 -3.8 +0.3
mixed community RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x +0.3 +0.5 -5.0 -4.6 +0.4
mixed community Fungal_growth_media_mCAFEs_v1 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL +0.3 -0.1 -4.5 -4.4 +0.3
mixed community RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x +0.0 +0.3 -5.4 -2.6 -0.6
mixed community Fungal_growth_media_mCAFEs_v1 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL +0.3 +0.5 -5.4 -3.8 +0.2
mixed community LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL +0.1 +0.6 -5.7 -4.2 +0.9
rhizosphere rhizosphere sample 1; outgrowth in LB +0.2 +0.2 -5.4 -3.4 +0.4
mixed community RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x +0.3 +0.2 -4.2 -4.3 +0.1
stress Fungal_growth_media_mCAFEs_v1 with nitrate 20 mM +0.3 +0.3 -3.8 -4.6 -0.2
supernatant Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml +0.1 +0.3 -3.8 -5.6 +1.2
mixed community RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x +0.3 +0.3 -4.1 -3.9 -0.4
mixed community LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL +0.1 +1.4 -4.8 -3.0 -0.5
stress Fungal_growth_media_mCAFEs_v1 with Vancomycin 0.01 mg/mL +0.5 +0.3 -3.3 -4.4 +0.3
mixed community LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL +0.0 +0.8 -5.3 -2.6 +0.5
nitrogen fixation control D-Glucose (C) with ammonium chloride; anaerobic; 8 days +0.2 +0.4 -4.6 -2.9 +0.6
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days +0.4 +0.1 -5.5 -2.0 +0.9
stress Sisomicin 0.008 mg/ml -0.2 -0.6 +3.4 +4.3 -0.4
stress Sisomicin 0.008 mg/ml +0.4 +0.8 +4.3 +5.1 -0.4
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