Fitness for 5 genes in Klebsiella michiganensis M5al

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500 ntBWI76_RS17245 and BWI76_RS17250 are separated by 478 nucleotidesBWI76_RS17250 and BWI76_RS17255 are separated by 176 nucleotidesBWI76_RS17255 and BWI76_RS17260 are separated by 144 nucleotidesBWI76_RS17260 and BWI76_RS17265 are separated by 93 nucleotides BWI76_RS17245: BWI76_RS17245 - hypothetical protein, at 3,573,050 to 3,573,697 _RS17245 BWI76_RS17250: BWI76_RS17250 - bifunctional acetaldehyde-CoA/alcohol dehydrogenase, at 3,574,176 to 3,576,851 _RS17250 BWI76_RS17255: BWI76_RS17255 - thymidine kinase, at 3,577,028 to 3,577,645 _RS17255 BWI76_RS17260: BWI76_RS17260 - hypothetical protein, at 3,577,790 to 3,578,108 _RS17260 BWI76_RS17265: BWI76_RS17265 - DNA-binding protein, at 3,578,202 to 3,578,609 _RS17265
Group Condition BWI76_RS17245 BWI76_RS17250 BWI76_RS17255 BWI76_RS17260 BWI76_RS17265
nitrogen fixation D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 3 days -0.1 -3.8 -0.3 +0.1 N.D.
nitrogen fixation control D-Glucose (C) with ammonium chloride; anaerobic; 8 days -0.1 -3.1 -0.1 +0.1 N.D.
nitrogen source RCH2_defined_Glucose_noNitrogen in Eppendorf tube with sand -0.2 -2.3 -0.6 -0.1 N.D.
mixed community RCH2_defined_Glucose_noNitrogen in Eppendorf tube with 0.1 mM glass beads, cultured with Sinorhizobium meliloti and Methylobacterium sp. UNC378MF -0.2 -2.2 -0.1 -0.2 N.D.
mixed community RCH2_defined_Glucose_noNitrogen in Eppendorf tube with sand, cultured with Sinorhizobium meliloti and Methylobacterium sp. UNC378MF +0.1 -2.2 -0.2 -0.2 N.D.
supernatant Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x -0.2 -0.6 -0.8 -0.6 N.D.
nitrogen source RCH2_defined_Glucose_noNitrogen in Eppendorf tube -0.2 -1.8 -0.3 -0.0 N.D.
nitrogen source RCH2_defined_Glucose_noNitrogen in Eppendorf tube with 0.1 mM glass beads -0.1 -1.5 -0.4 -0.1 N.D.
supernatant Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 1x +0.1 -0.9 -0.8 -0.5 N.D.
rhizosphere rhizosphere sample 1; outgrowth in LB -0.3 +0.3 -0.9 -1.1 N.D.
supernatant Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.67x; and D-Glucose 37 mM -0.1 -1.0 -0.8 -0.1 N.D.
supernatant Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x; and D-Glucose 55.5 mM +0.0 -0.7 -0.7 -0.4 N.D.
supernatant RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x; and D-Glucose 20mM -0.1 -0.8 -0.6 -0.2 N.D.
supernatant RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x +0.1 -0.8 -0.7 -0.4 N.D.
supernatant Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM +0.0 -0.9 -0.7 -0.2 N.D.
nitrogen fixation D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 8 days -0.0 -1.5 -0.3 +0.1 N.D.
supernatant Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.67x; and D-Glucose 37 mM +0.2 -1.0 -0.7 +0.1 N.D.
exudates RCH2_defined_noCarbon with Brachypodium distachyon 27-day exudates 3 x -0.3 +0.4 -0.9 -0.5 N.D.
phage KLEB17 MOI 0.1 -0.6 +0.6 -0.0 -0.8 N.D.
phage K. M5a1 KLEB22 MOI 10 +0.3 +0.5 -0.6 -0.8 N.D.
nitrogen source Thymine (N) -0.0 +0.7 -0.8 -0.5 N.D.
nitrogen source L-Lysine (N) -0.1 +0.5 +0.2 -1.1 N.D.
mixed community RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x +0.2 +0.6 -0.2 -0.9 N.D.
stress RCH2_defined_1percent_glucose with Chloride 600 mM +0.3 -1.0 +0.4 +0.7 N.D.
phage K. M5a1 KLEB23 MOI 1 +0.6 +0.6 -0.6 +0.2 N.D.
phage KLEB29 MOI 1 -0.1 +0.7 +0.7 +0.4 N.D.
stress Cephalothin 0.16 mg/ml -0.0 +0.5 +0.9 +0.4 N.D.
carbon source Gly-Glu (C) +0.1 +0.1 +0.7 +1.1 N.D.
phage KLEB31 MOI 1 +0.3 +0.7 +0.6 +0.4 N.D.
stress Cobalt chloride 0.5 mM +0.8 +1.0 +0.3 +0.8 N.D.
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