Fitness for 5 genes in Klebsiella michiganensis M5al

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500 ntBWI76_RS17090 and BWI76_RS17095 are separated by 81 nucleotidesBWI76_RS17095 and BWI76_RS17100 overlap by 1 nucleotidesBWI76_RS17100 and BWI76_RS17105 are separated by 224 nucleotidesBWI76_RS17105 and BWI76_RS17110 are separated by 24 nucleotides BWI76_RS17090: BWI76_RS17090 - NLP/P60 protein, at 3,546,764 to 3,547,228 _RS17090 BWI76_RS17095: BWI76_RS17095 - vitamin B12 ABC transporter ATP-binding protein BtuD, at 3,547,310 to 3,548,059 _RS17095 BWI76_RS17100: BWI76_RS17100 - glutathione peroxidase, at 3,548,059 to 3,548,610 _RS17100 BWI76_RS17105: BWI76_RS17105 - methionine ABC transporter substrate-binding protein MetQ, at 3,548,835 to 3,549,635 _RS17105 BWI76_RS17110: BWI76_RS17110 - vitamin B12 ABC transporter permease BtuC, at 3,549,660 to 3,550,646 _RS17110
Group Condition BWI76_RS17090 BWI76_RS17095 BWI76_RS17100 BWI76_RS17105 BWI76_RS17110
phage KLEB17 MOI 1 N.D. -0.4 +0.1 -0.0 -5.1
supernatant RCH2_defined_glucose with Supernatant; Trichoderma harzianum M10 and Trichoderma harzanium TH1 and Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1x N.D. -1.1 +0.1 +0.1 -2.8
nitrogen source Glycine (N) N.D. -1.6 -0.1 -0.0 -1.7
phage KLEB1 MOI 0.1 N.D. -0.3 -0.5 -0.6 -1.8
carbon source 2-Deoxyadenosine 5-monophosphate 5 mM (C) N.D. -0.7 -2.4 +0.4 -0.3
phage KLEB1 MOI 10 N.D. -0.6 +0.2 -1.0 -1.6
supernatant RCH2_defined_glucose with Supernatant; Trichoderma virens RS grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1x N.D. -0.6 -0.4 -0.2 -1.1
nitrogen source L-Asparagine (N) N.D. -0.7 -0.3 +0.0 -1.3
supernatant RCH2_defined_glucose with Supernatant; Trichoderma harzianum M10 and Trichoderma harzanium TH1 and Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1x N.D. -0.8 -0.3 +0.1 -1.1
nitrogen source Adenosine (N) N.D. -0.9 -0.3 +0.2 -1.1
supernatant RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x N.D. -0.8 +0.3 -0.3 -1.3
phage KLEB31 MOI 1 N.D. -0.1 -0.1 +0.3 -2.1
carbon source 2-Deoxy-D-ribonic lithium salt 10 mM (C) N.D. -0.6 +0.4 -0.1 -1.5
mixed community RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x N.D. -1.2 +0.3 -0.1 -0.7
mixed community LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL N.D. -0.4 +0.4 -0.2 -1.3
mixed community RCH2_defined_noCarbon with Mixed culture; Dyella japonica UNC79MFTsu3.2 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x N.D. -0.8 +0.7 -0.3 -1.2
nitrogen source Casamino Acids (N) N.D. +0.1 -0.5 +0.3 -1.4
nitrogen source Uridine (N) N.D. +0.9 -1.6 -0.1 -0.6
mixed community RCH2_defined_noCarbon with Mixed culture; Pseudomonas simiae WCS417 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x N.D. -1.0 +0.5 -0.2 -0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. +0.9 -0.2 -0.3 -1.7
exudates RCH2_defined_noCarbon with Brachypodium distachyon 27-day exudates 3 x N.D. +0.3 +1.2 -1.0 -1.0
stress Cobalt chloride 0.5 mM N.D. -0.4 +0.8 +0.2 -1.0
stress Cephalothin 0.16 mg/ml N.D. +0.4 +0.7 +0.1 -1.4
phage K. M5a1 KLEB26 MOI 0.1 N.D. +0.6 -1.4 +1.0 -0.1
supernatant RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.67x N.D. +0.1 -0.9 +0.1 +1.4
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days N.D. +0.4 +1.2 -0.2 -0.5
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -0.1 +2.3 +0.0 -1.3
nitrogen source Uridine (N) N.D. +0.9 +0.0 -0.5 +1.2
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. +0.9 +0.7 +0.2 +0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. +0.4 +0.7 +0.1 +2.7
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