Fitness for 5 genes in Klebsiella michiganensis M5al

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500 ntBWI76_RS16735 and BWI76_RS16740 are separated by 48 nucleotidesBWI76_RS16740 and BWI76_RS16745 are separated by 106 nucleotidesBWI76_RS16745 and BWI76_RS16750 are separated by 10 nucleotidesBWI76_RS16750 and BWI76_RS16755 are separated by 23 nucleotides BWI76_RS16735: BWI76_RS16735 - cold-shock protein, at 3,467,232 to 3,467,444 _RS16735 BWI76_RS16740: BWI76_RS16740 - transcriptional regulator, at 3,467,493 to 3,468,365 _RS16740 BWI76_RS16745: BWI76_RS16745 - alpha-acetolactate decarboxylase, at 3,468,472 to 3,469,251 _RS16745 BWI76_RS16750: BWI76_RS16750 - acetolactate synthase, at 3,469,262 to 3,470,941 _RS16750 BWI76_RS16755: BWI76_RS16755 - acetoin reductase, at 3,470,965 to 3,471,735 _RS16755
Group Condition BWI76_RS16735 BWI76_RS16740 BWI76_RS16745 BWI76_RS16750 BWI76_RS16755
phage K. M5a1 KLEB26 MOI 0.1 +0.2 -0.7 -0.5 -1.0 -1.6
phage KLEB17 MOI 1 -1.2 -0.9 -0.1 -0.2 -0.4
phage K. M5a1 KLEB23 MOI 1 -0.3 -0.3 -0.4 -0.3 -1.1
supernatant Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x +0.2 -0.7 -0.8 -0.9 -0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.0 -0.7 -0.9 +0.4 -0.7
nitrogen source D-Alanine (N) -1.1 +0.1 +0.4 +0.1 -0.7
phage KLEB1 MOI 10 +1.0 -0.5 -0.2 -0.2 -1.4
supernatant Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 1x +0.6 +0.1 +0.1 -0.5 -1.3
nitrogen fixation D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 3 days -0.9 +0.5 +0.0 +0.2 -0.8
mixed community RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x -1.3 -0.5 +0.4 +0.7 +0.2
stress RCH2_defined_1percent_glucose with Chloride 600 mM +0.2 +0.3 +0.7 -1.1 -0.3
mixed community RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x -1.7 +0.2 +0.4 +0.3 +0.8
mixed community Fungal_growth_media_mCAFEs_v1 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL -0.8 +0.3 +0.7 +0.4 -0.4
mixed community Fungal_growth_media_mCAFEs_v1 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL -0.9 -0.3 +0.7 +0.5 +0.1
mixed community LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL -1.3 -0.0 +0.4 +0.4 +0.8
exudates RCH2_defined_noCarbon with Brachypodium distachyon 27-day exudates 3 x -0.8 -0.4 +0.5 +0.5 +0.5
mixed community RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x +0.5 -1.0 +0.7 +0.3 -0.1
rhizosphere rhizosphere sample 1; outgrowth in LB -0.8 +0.4 +0.7 -0.1 +0.5
stress Fungal_growth_media_mCAFEs_v1 with Phosphomycin 0.01 mg/mL +0.3 +0.7 +0.8 -0.3 -0.5
mixed community LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL -0.9 +0.4 +0.3 +0.7 +0.5
nitrogen source L-Cysteine (N) -0.7 +0.7 +0.2 +0.7 +0.3
supernatant RCH2_defined_glucose with Supernatant; Trichoderma harzianum M10 and Trichoderma harzanium TH1 and Trichoderma virens G41 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1 x and 2,2'-Dipyridyl 50 uM -0.7 +0.2 +0.5 +0.6 +0.5
mixed community LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL -0.1 -0.8 +0.8 +0.4 +0.9
stress Fungal_growth_media_mCAFEs_v1 with Zinc 2 mM -1.3 +0.2 +0.6 +0.9 +1.2
mixed community RCH2_defined_noCarbon with Mixed culture; Arthrobacter sp. AnTP99 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x -0.3 -0.3 +0.9 +0.7 +0.8
mixed community Fungal_growth_media_mCAFEs_v1 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL +0.5 +0.2 +0.7 +0.7 -0.4
supernatant Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 1x -0.2 +0.5 +0.7 +0.6 +0.6
supernatant Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x +1.0 +0.1 +0.5 +0.5 +0.6
mixed community LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL +0.9 +0.1 +0.5 +0.8 +0.6
supernatant Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml +1.3 -0.2 +0.9 +0.5 +0.6
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