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Fitness for 5 genes in
Klebsiella michiganensis M5al
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500 nt
BWI76_RS16435 and BWI76_RS16440 are separated by 171 nucleotides
BWI76_RS16440 and BWI76_RS16445 are separated by 10 nucleotides
BWI76_RS16445 and BWI76_RS16450 overlap by 1 nucleotides
BWI76_RS16450 and BWI76_RS16455 overlap by 8 nucleotides
BWI76_RS16435: BWI76_RS16435 - SlyA family transcriptional regulator, at 3,409,763 to 3,410,203
_RS16435
BWI76_RS16440: BWI76_RS16440 - DUF1656 domain-containing protein, at 3,410,375 to 3,410,611
_RS16440
BWI76_RS16445: BWI76_RS16445 - auxiliary transporter membrane fusion protein, at 3,410,622 to 3,411,518
_RS16445
BWI76_RS16450: BWI76_RS16450 - fusaric acid resistance protein, at 3,411,518 to 3,413,542
_RS16450
BWI76_RS16455: BWI76_RS16455 - superoxide dismutase, at 3,413,535 to 3,414,056
_RS16455
Group
Condition
BWI76
_RS16435
BWI76
_RS16440
BWI76
_RS16445
BWI76
_RS16450
BWI76
_RS16455
phage
K. M5a1 KLEB26 MOI 0.1
N.D.
N.D.
+1.3
+0.5
-4.1
stress
Fungal_growth_media_mCAFEs_v1 with Zinc 2 mM
N.D.
N.D.
-0.6
-0.0
-1.6
mixed community
LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL
N.D.
N.D.
-0.4
-0.7
-1.0
phage
KLEB1 MOI 10
N.D.
N.D.
+1.2
+0.6
-3.9
phage
K. M5a1 KLEB23 MOI 10
N.D.
N.D.
-0.7
+0.4
-1.8
phage
KLEB17 MOI 0.1
N.D.
N.D.
-0.3
+0.4
-2.0
phage
KLEB30 MOI 1
N.D.
N.D.
-0.6
+0.2
-1.4
agar plate interaction control
Parafilmed volatile agar plate with no fungus
N.D.
N.D.
+0.2
+0.5
-2.4
supernatant
LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
N.D.
N.D.
-1.1
+0.3
-0.8
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
N.D.
N.D.
-1.8
+0.4
-0.0
phage
K. M5a1 KLEB10 MOI 10
N.D.
N.D.
+0.5
-0.1
-1.5
supernatant
Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM
N.D.
N.D.
-0.1
+0.5
-1.4
mixed community
RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
N.D.
N.D.
-0.9
-0.4
+0.8
ecofab
LB in EcoFAB_10mL
N.D.
N.D.
+0.7
+0.0
-1.1
agar plate interaction
Taped volatile agar plate with Trichoderma atroviridae IMI
N.D.
N.D.
-0.7
-0.5
+1.4
stress
Cobalt chloride 0.5 mM
N.D.
N.D.
-0.1
-0.8
+1.1
nitrogen source
L-Isoleucine (N)
N.D.
N.D.
-0.8
+0.3
+1.0
carbon source
2-Deoxyadenosine 4 mM (C)
N.D.
N.D.
-0.4
-0.2
+1.4
nitrogen source
Uridine (N)
N.D.
N.D.
-0.2
+0.3
+1.7
phage
KLEB1 MOI 10
N.D.
N.D.
+0.8
-0.3
+1.3
supernatant
RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.67x
N.D.
N.D.
-0.5
+0.2
+2.2
phage
K. M5a1 KLEB10 MOI 1
N.D.
N.D.
+0.9
+0.4
+0.6
phage
K. M5a1 KLEB23 MOI 1
N.D.
N.D.
+0.5
+0.5
+1.1
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
N.D.
N.D.
+0.9
+1.2
-0.0
mixed community
RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
N.D.
N.D.
+0.5
+0.5
+1.2
mixed community
RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
N.D.
N.D.
+1.0
-0.1
+1.3
stress
copper (II) chloride 3 mM
N.D.
N.D.
+1.1
+0.5
+0.5
nitrogen source
N-Acetyl-D-glucosamine (N)
N.D.
N.D.
+0.9
+0.6
+0.8
phage
KLEB15 MOI 10
N.D.
N.D.
+1.3
+0.3
+0.8
rhizosphere
rhizosphere EcoFAB sample 3; outgrowth in LB
N.D.
N.D.
+2.7
-0.3
+0.7
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