Fitness for 5 genes in Klebsiella michiganensis M5al

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500 ntBWI76_RS16045 and BWI76_RS16050 are separated by 2 nucleotidesBWI76_RS16050 and BWI76_RS16055 overlap by 4 nucleotidesBWI76_RS16055 and BWI76_RS16060 overlap by 11 nucleotidesBWI76_RS16060 and BWI76_RS16065 are separated by 215 nucleotides BWI76_RS16045: BWI76_RS16045 - transporter, at 3,326,687 to 3,327,646 _RS16045 BWI76_RS16050: BWI76_RS16050 - malonate decarboxylase holo-ACP synthase, at 3,327,649 to 3,328,269 _RS16050 BWI76_RS16055: BWI76_RS16055 - malonate decarboxylase subunit epsilon, at 3,328,266 to 3,329,168 _RS16055 BWI76_RS16060: BWI76_RS16060 - LysR family transcriptional regulator, at 3,329,158 to 3,330,084 _RS16060 BWI76_RS16065: BWI76_RS16065 - malate:quinone oxidoreductase, at 3,330,300 to 3,331,955 _RS16065
Group Condition BWI76_RS16045 BWI76_RS16050 BWI76_RS16055 BWI76_RS16060 BWI76_RS16065
phage K. M5a1 KLEB23 MOI 10 N.D. -0.5 -1.1 -0.2 -0.3
rhizosphere rhizosphere EcoFAB sample 3; outgrowth in LB N.D. +0.6 +0.3 +0.1 -1.1
supernatant Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml N.D. -0.1 +0.7 -0.6 -0.1
carbon source L-Malic (C) N.D. +0.6 +0.4 -0.1 -0.7
phage KLEB29 MOI 1 N.D. +0.5 +0.4 -0.7 +0.0
mixed community LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL N.D. -0.5 +0.7 +0.3 -0.2
mixed community Fungal_growth_media_mCAFEs_v1 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL N.D. +0.1 +0.9 -0.3 -0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant N.D. -0.4 +0.9 -0.4 +0.2
carbon source 2'-Deoxyinosine 5 mM (C) N.D. -0.7 +0.4 +0.6 -0.1
mixed community LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL N.D. +0.5 +0.6 -0.5 -0.3
mixed community LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL N.D. +0.2 +0.8 -0.3 -0.3
nitrogen source Uridine (N) N.D. -0.4 +0.8 +0.5 -0.4
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. +1.0 -0.1 -0.4 +0.1
mixed community RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x N.D. +0.5 +0.7 -0.2 -0.2
mixed community RCH2_defined_noCarbon with Mixed culture; Cupriavidus sp. AnTP704 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x N.D. +0.6 +0.7 +0.0 -0.5
mixed community RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x N.D. +0.6 +0.6 -0.2 -0.2
carbon source RCH2_defined_noCarbon with Glu_DLac_Acetate_mix 0.1 x N.D. +0.5 +0.8 +0.2 -0.2
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days N.D. +0.8 +0.8 +0.2 -0.5
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -0.1 +1.4 +0.1 -0.0
mixed community LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL N.D. +0.4 +1.0 +0.5 -0.5
mixed community RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x N.D. +0.3 +0.8 +0.4 -0.1
carbon source D,L-Malic Acid (C) N.D. +1.1 +1.6 -0.6 -0.5
mixed community Fungal_growth_media_mCAFEs_v1 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL N.D. +0.2 +0.8 +0.4 +0.1
mixed community RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x N.D. +0.2 +1.0 +0.3 +0.0
stress Sisomicin 0.008 mg/ml N.D. +0.7 +0.3 +0.1 +0.5
mixed community LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL N.D. +0.7 +1.1 +0.1 -0.2
carbon source Gly-Glu (C) N.D. +1.7 +0.2 -0.5 +0.2
phage KLEB17 MOI 1 N.D. +0.8 +0.6 +0.5 -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. +1.0 +0.1 +0.5 +0.3
carbon source D,L-Malic Acid (C) N.D. +1.2 +1.5 -0.1 -0.4
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