Fitness for 5 genes in Klebsiella michiganensis M5al

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500 ntBWI76_RS15020 and BWI76_RS15025 are separated by 29 nucleotidesBWI76_RS15025 and BWI76_RS15030 overlap by 4 nucleotidesBWI76_RS15030 and BWI76_RS15035 overlap by 16 nucleotidesBWI76_RS15035 and BWI76_RS15040 are separated by 31 nucleotides BWI76_RS15020: BWI76_RS15020 - 2-oxobutyrate oxidase, at 3,144,595 to 3,145,623 _RS15020 BWI76_RS15025: BWI76_RS15025 - NLPA lipoprotein, at 3,145,653 to 3,146,459 _RS15025 BWI76_RS15030: BWI76_RS15030 - ABC transporter, at 3,146,456 to 3,147,247 _RS15030 BWI76_RS15035: BWI76_RS15035 - metal ABC transporter permease, at 3,147,232 to 3,147,903 _RS15035 BWI76_RS15040: BWI76_RS15040 - putative 2-nitropropane dioxygenase, at 3,147,935 to 3,148,981 _RS15040
Group Condition BWI76_RS15020 BWI76_RS15025 BWI76_RS15030 BWI76_RS15035 BWI76_RS15040
supernatant control Vogels_fungal_media; pH 5.5 -0.9 N.D. -0.3 -2.1 -0.2
mixed community RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x -0.1 N.D. +0.3 -3.6 -0.1
nitrogen source Uridine (N) +0.5 N.D. -0.3 -3.3 +0.1
phage K. M5a1 KLEB26 MOI 0.1 -0.1 N.D. -0.4 -2.7 +0.2
stress nitrite 20 mM -0.0 N.D. -0.8 -1.6 -0.2
supernatant control:fungal media R2A with Vogels_fungal_media 0.8X -0.6 N.D. -0.7 -1.1 -0.1
nitrogen source Thymidine (N) +0.2 N.D. -0.4 -2.2 +0.1
phage KLEB29 MOI 1 +0.4 N.D. -0.9 -1.0 -0.5
mixed community LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL -1.1 N.D. -0.6 -0.5 +0.2
nitrogen source L-Citrulline (N) +0.2 N.D. -1.0 -1.1 +0.0
carbon source D-Trehalose (C) -0.1 N.D. +0.2 -1.8 -0.1
phage KLEB13 MOI 0.1 +0.7 N.D. -1.4 -1.1 +0.1
supernatant LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x +0.2 N.D. +0.3 -1.7 -0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.3 N.D. +0.1 -1.9 -0.0
phage K. M5a1 KLEB23 MOI 1 +0.4 N.D. -0.3 -1.0 -0.6
nitrogen source N-Acetyl-D-glucosamine (N) +0.4 N.D. -0.3 -1.8 +0.3
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.9 N.D. -0.6 -1.0 -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.5 N.D. -0.9 +0.7 +0.1
stress Chloramphenicol 0.001 mg/ml +1.1 N.D. -0.6 -0.7 -0.1
nitrogen source L-aspartic (N) +0.6 N.D. +0.0 -1.4 +0.4
micoeukaryotes Tetrahymena sp., Mixed culture-1 +0.9 N.D. -0.5 +0.7 -0.1
stress Fungal_growth_media_mCAFEs_v1 with Phosphomycin 0.01 mg/mL -0.4 N.D. +0.7 +0.9 +0.2
supernatant RCH2_defined_glucose with Supernatant; Trichoderma virens RS grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1x -0.0 N.D. -0.3 +1.4 +0.5
phage KLEB26 MOI0.1 +0.5 N.D. -0.2 +1.4 +0.1
phage KLEB16 MOI 1 -0.3 N.D. +0.3 +1.6 +0.3
phage KLEB15 MOI 0.1 +0.7 N.D. -0.1 +1.0 +0.6
phage KLEB30 MOI 1 +0.5 N.D. +0.0 +1.8 -0.1
supernatant Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x +0.4 N.D. +0.9 +0.7 +0.7
mixed community RCH2_defined_noCarbon with Mixed culture; Escherichia coli BW25113 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x +0.1 N.D. +0.7 +2.0 +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.1 N.D. -0.3 +4.2 +0.4
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